PMID- 33582911
OWN - NLM
STAT- MEDLINE
DCOM- 20211006
LR  - 20211006
IS  - 2523-899X (Electronic)
IS  - 2523-899X (Linking)
VI  - 41
IP  - 1
DP  - 2021 Feb
TI  - Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhance Insulin 
      Sensitivity in Insulin Resistant Human Adipocytes.
PG  - 87-93
LID - 10.1007/s11596-021-2323-4 [doi]
AB  - Insulin resistance is an essential characteristic of type 2 diabetes mellitus 
      (T2DM), which can be induced by glucotoxicity and adipose chronic inflammation. 
      Mesenchymal stem cells (MSCs) and their exosomes were reported to ameliorate T2DM 
      and its complications by their immunoregulatory and healing abilities. Exosomes 
      derived from MSCs contain abundant molecules to mediate crosstalk between cells 
      and mimic biological function of MSCs. But the role of exosomes derived from 
      human umbilical cord mesenchymal stem cells (hUC-MSCs) in insulin resistance of 
      human adipocytes is unclear. In this study, exosomes were harvested from the 
      conditioned medium of hUC-MSCs and added to insulin-resistant adipocytes. 
      Insulin-stimulated glucose uptake was measured by glucose oxidase/peroxidase 
      assay. The signal pathway involved in exosome-treated adipocytes was detected by 
      RT-PCR and Western blotting. The biological characteristics and function were 
      compared between hUC-MSCs and human adipose-derived mesenchymal stem cells 
      (hAMSCs). The results showed that hAMSCs had better adipogenic ability than 
      hUC-MSCs. After induction of mature adipocytes by adipogenesis of hAMSC, the 
      model of insulin-resistant adipocytes was successfully established by TNF-alpha and 
      high glucose intervention. After exosome treatment, the insulin-stimulated 
      glucose uptake was significantly increased. In addition, the effect of exosomes 
      could be stabilized for at least 48 h. Furthermore, the level of leptin was 
      significantly decreased, and the mRNA expression of sirtuin-1 and insulin 
      receptor substrate-1 was significantly upregulated after exosome treatment. In 
      conclusion, exosomes significantly improve insulin sensitivity in 
      insulin-resistant human adipocytes, and the mechanism involves the regulation of 
      adipokines.
FAU - Chen, Mei-Ting
AU  - Chen MT
AD  - Department of Endocrinology, Key Laboratory of Endocrinology, Ministry of Health, 
      Peking Union Medical College Hospital, Diabetes Research Center of Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, 
      China.
AD  - Department of Medical Oncology, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, 510060, China.
FAU - Zhao, Yi-Ting
AU  - Zhao YT
AD  - Department of PET-CT Center, Cancer Hospital, Chinese Academy of Medical Sciences 
      & Peking Union Medical College, Beijing, 100021, China.
FAU - Zhou, Li-Yuan
AU  - Zhou LY
AD  - Department of Endocrinology, Key Laboratory of Endocrinology, Ministry of Health, 
      Peking Union Medical College Hospital, Diabetes Research Center of Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, 
      China.
FAU - Li, Ming
AU  - Li M
AD  - Department of Endocrinology, Key Laboratory of Endocrinology, Ministry of Health, 
      Peking Union Medical College Hospital, Diabetes Research Center of Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, 
      China.
FAU - Zhang, Qian
AU  - Zhang Q
AD  - Department of Endocrinology, Key Laboratory of Endocrinology, Ministry of Health, 
      Peking Union Medical College Hospital, Diabetes Research Center of Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, 
      China.
FAU - Han, Qin
AU  - Han Q
AD  - Center of Excellence in Tissue Engineering, Key Laboratory of Beijing, Institute 
      of Basic Medical Sciences and School of Basic Medicine, Chinese Academy of 
      Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
FAU - Xiao, Xin-Hua
AU  - Xiao XH
AD  - Department of Endocrinology, Key Laboratory of Endocrinology, Ministry of Health, 
      Peking Union Medical College Hospital, Diabetes Research Center of Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, 
      China. xiaoxh2014@vip.163.com.
LA  - eng
PT  - Journal Article
DEP - 20210213
PL  - China
TA  - Curr Med Sci
JT  - Current medical science
JID - 101729993
RN  - 0 (Insulin)
RN  - 0 (Leptin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipocytes/cytology/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Exosomes/*metabolism
MH  - Glucose/metabolism
MH  - Humans
MH  - Insulin/metabolism
MH  - *Insulin Resistance
MH  - Leptin/metabolism
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Sirtuin 1/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - exosome
OT  - hyperglycemia injury
OT  - insulin resistance
OT  - mesenchymal stem cells
OT  - type 2 diabetes mellitus
EDAT- 2021/02/15 06:00
MHDA- 2021/10/07 06:00
CRDT- 2021/02/14 20:38
PHST- 2019/10/07 00:00 [received]
PHST- 2020/11/19 00:00 [accepted]
PHST- 2021/02/14 20:38 [entrez]
PHST- 2021/02/15 06:00 [pubmed]
PHST- 2021/10/07 06:00 [medline]
AID - 10.1007/s11596-021-2323-4 [pii]
AID - 10.1007/s11596-021-2323-4 [doi]
PST - ppublish
SO  - Curr Med Sci. 2021 Feb;41(1):87-93. doi: 10.1007/s11596-021-2323-4. Epub 2021 Feb 
      13.