PMID- 33583303
OWN - NLM
STAT- MEDLINE
DCOM- 20220103
LR  - 20220715
IS  - 1744-8387 (Electronic)
IS  - 1478-9450 (Linking)
VI  - 18
IP  - 1
DP  - 2021 Jan
TI  - Differential levels of CHMP2B, LLPH, and SLC25A51 proteins in secondary renal 
      amyloidosis.
PG  - 65-73
LID - 10.1080/14789450.2021.1890588 [doi]
AB  - OBJECTIVES: Renal amyloidosis (RA) is a rare disease, typically manifested with 
      proteinuria, nephrotic syndrome, and ultimately leads to renal failure. The 
      present study aims to profile the proteomes of renal amyloidosis patient's serum 
      and healthy controls, along with relative quantification to find out robust 
      markers for RA. METHODS: In this study, 12 RA patients and their corresponding 
      age and gender-matched healthy controls were recruited from the Nephrology 
      department of Max Super Specialty Hospital, New Delhi. We employed gel-based 
      proteomic approach coupled with MALDI-TOF MS to compare protein expression 
      patterns in RA patients and controls. Furthermore, validation of differential 
      proteins (selected) was done using bio-layer interferometry. RESULTS: Eleven 
      proteins showed remarkably altered expression levels. Moreover, expression 
      modulation of three proteins (LLPH, SLC25A51, and CHMP2B) was validated which 
      corroborated with two-dimensional gel electrophoresis (2-DE) results showing 
      significant upregulation (p < 0.05) in RA patients followed by ROC analysis which 
      demonstrated the diagnostic potential of these proteins. A protein-protein master 
      network was generated implicating the above identified proteins along with their 
      interactors, fishing out the routes leading to amyloidosis. CONCLUSION: This 
      study indicates that the identified serum proteomic signatures could improve 
      early diagnosis and lead to possible therapeutic targets in RA.
FAU - Gupta, Nimisha
AU  - Gupta N
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, 
      New Delhi, INDIA.
FAU - Sahar, Tahreem
AU  - Sahar T
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, 
      New Delhi, INDIA.
FAU - Khowal, Sapna
AU  - Khowal S
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, 
      New Delhi, INDIA.
FAU - Ganaie, Ishfaq Ahmad
AU  - Ganaie IA
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, 
      New Delhi, INDIA.
FAU - Mughees, Mohd
AU  - Mughees M
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, 
      New Delhi, INDIA.
FAU - Khullar, Dinesh
AU  - Khullar D
AD  - Department of Nephrology Nephrology and Renal Transplant Medicine, Max Super 
      Speciality Hospital (A Unit of Devki Devi Foundation), New Delhi, INDIA.
FAU - Jain, S K
AU  - Jain SK
AD  - Department of Biochemistry, Hamdard Institute of Medical Sciences and Research, 
      Jamia Hamdard, New Delhi, INDIA.
FAU - Wajid, Saima
AU  - Wajid S
AUID- ORCID: 0000-0003-1558-8233
AD  - Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, 
      New Delhi, INDIA.
LA  - eng
SI  - figshare/10.6084/m9.figshare.14159374.v1
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210304
PL  - England
TA  - Expert Rev Proteomics
JT  - Expert review of proteomics
JID - 101223548
RN  - 0 (CHMP2B protein, human)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (LLPH protein, human)
RN  - 0 (Mitochondrial Membrane Transport Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteome)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (SLC25A51 protein, human)
SB  - IM
MH  - Amyloidosis/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Endosomal Sorting Complexes Required for Transport/*metabolism
MH  - Female
MH  - Humans
MH  - Kidney Diseases/*metabolism
MH  - Male
MH  - Mitochondrial Membrane Transport Proteins/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Proteome/analysis/metabolism
MH  - *Proteomics
MH  - RNA-Binding Proteins/*metabolism
MH  - Rare Diseases/metabolism
OTO - NOTNLM
OT  - 2D gel electrophoresis
OT  - MALDI-TOF MS
OT  - bio-layer interferometry
OT  - early diagnosis
OT  - pathogenesis
OT  - serum biomarkers
EDAT- 2021/02/16 06:00
MHDA- 2022/01/04 06:00
CRDT- 2021/02/15 05:34
PHST- 2021/02/16 06:00 [pubmed]
PHST- 2022/01/04 06:00 [medline]
PHST- 2021/02/15 05:34 [entrez]
AID - 10.1080/14789450.2021.1890588 [doi]
PST - ppublish
SO  - Expert Rev Proteomics. 2021 Jan;18(1):65-73. doi: 10.1080/14789450.2021.1890588. 
      Epub 2021 Mar 4.