PMID- 33586729
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20210622
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 12
IP  - 5
DP  - 2021 Mar 15
TI  - The polysaccharides from the Grifola frondosa fruiting body prevent 
      lipopolysaccharide/D-galactosamine-induced acute liver injury via the 
      miR-122-Nrf2/ARE pathways.
PG  - 1973-1982
LID - 10.1039/d0fo03327h [doi]
AB  - Polysaccharides can be used as a potential hepatoprotective agent in the 
      treatment of acute liver injury. However, the underlying mechanism governing how 
      polysaccharides protect against acute liver injury induced by 
      lipopolysaccharide/d-galactosamine (LPS/d-GalN) remains unclear. To investigate 
      the mechanism, the anti-oxidative and anti-inflammatory action and pathways were 
      determined. In this study, we investigated the hepatoprotective effects of 
      Grifola frondosa polysaccharides (GFP), which are obtained from the fruiting body 
      of Grifola frondosa, on (LPS/d-GalN)-induced liver injury in mice. 
      Histopathological analyses showed that GFP protected against LPS/d-GalN-induced 
      lung inflammation. The activities of alanine aminotransferase (ALT) and aspartate 
      aminotransferase (AST) and the levels of the inflammatory mediators tumor 
      necrosis factor-alpha (TNF-alpha), interleukin (IL)-2, IL-6, and monocyte chemoattractant 
      protein-1 (MCP-1) were inhibited by GFP. The LPS/d-GalN-induced myeloperoxidase 
      (MPO) activity and malondialdehyde (MDA) content were inhibited by GFP. The 
      levels of superoxide dismutase (SOD) and glutathione (GSH) were upregulated by 
      GFP. The GFP-treated group showed reduced expression levels of miR-122 in liver 
      tissue. Nrf2 has been identified as a potential target of miR-122. The western 
      blotting results showed that GFP attenuates LPS/d-GalN-induced acute liver injury 
      via upregulating transcription factors Nrf2, Nqo-1, and HO-1 and downregulating 
      transcription factor Keap-1 in the Nrf2/ARE signaling pathway. In conclusion, 
      these results indicated that GFP was highly effective in inhibiting liver injury 
      and may be a promising potential therapeutic reagent for liver injury treatment. 
      GFP exerts protective effects against LPS/d-GalN-induced liver injury in mice, 
      which may be related to the regulation of the miR-122-Nrf2/ARE pathways.
FAU - Meng, Meng
AU  - Meng M
AUID- ORCID: 0000-0002-6499-5452
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education, College of food Engineering and 
      Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, 
      Tianjin Economy Technological Development Area, Tianjin 300457, People Republic 
      of China. lhhou@tust.edu.cn.
FAU - Zhang, Rui
AU  - Zhang R
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education, College of food Engineering and 
      Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, 
      Tianjin Economy Technological Development Area, Tianjin 300457, People Republic 
      of China. lhhou@tust.edu.cn.
FAU - Han, Ran
AU  - Han R
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education, College of food Engineering and 
      Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, 
      Tianjin Economy Technological Development Area, Tianjin 300457, People Republic 
      of China. lhhou@tust.edu.cn.
FAU - Kong, Yu
AU  - Kong Y
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education, College of food Engineering and 
      Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, 
      Tianjin Economy Technological Development Area, Tianjin 300457, People Republic 
      of China. lhhou@tust.edu.cn.
FAU - Wang, Ruhua
AU  - Wang R
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education, College of food Engineering and 
      Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, 
      Tianjin Economy Technological Development Area, Tianjin 300457, People Republic 
      of China. lhhou@tust.edu.cn.
FAU - Hou, Lihua
AU  - Hou L
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education, College of food Engineering and 
      Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, 
      Tianjin Economy Technological Development Area, Tianjin 300457, People Republic 
      of China. lhhou@tust.edu.cn.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Antioxidants)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn122 microRNA, mouse)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Polysaccharides)
RN  - 7535-00-4 (Galactosamine)
SB  - IM
MH  - Animals
MH  - Antioxidant Response Elements/genetics
MH  - Antioxidants/*pharmacology
MH  - Chemical and Drug Induced Liver Injury/*metabolism
MH  - Galactosamine/adverse effects
MH  - Grifola/*chemistry
MH  - Lipopolysaccharides/adverse effects
MH  - Liver/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/metabolism
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Polysaccharides/*pharmacology
EDAT- 2021/02/16 06:00
MHDA- 2021/06/23 06:00
CRDT- 2021/02/15 08:44
PHST- 2021/02/16 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2021/02/15 08:44 [entrez]
AID - 10.1039/d0fo03327h [doi]
PST - ppublish
SO  - Food Funct. 2021 Mar 15;12(5):1973-1982. doi: 10.1039/d0fo03327h.