PMID- 33586798 OWN - NLM STAT- MEDLINE DCOM- 20210708 LR - 20231213 IS - 1745-4514 (Electronic) IS - 0145-8884 (Linking) VI - 45 IP - 4 DP - 2021 Apr TI - Therapeutic effect of ursolic acid on fetal development in pregnant rats with gestational diabetes mellitus via AGEs-RAGE signaling pathway. PG - e13651 LID - 10.1111/jfbc.13651 [doi] AB - To investigate the effect of ursolic acid on the fetal development of gestational diabetes mellitus (GDM) caused by streptozotocin (STZ) and explore the potential mechanism for it. For the current experimental research, SD rats (pregnant animal) were used. STZ has been used to cause the diabetes mellitus in pregnant rats. Rats with evolved GDM were randomly divided and ursolic acid was given to pregnant rats in the experimental period up to 19 days in a dose-dependent manner. Blood samples and fetal rats of all group rats were collected at 19 days (pregnant rats), fetal rats and placental rats were weighted and the blood glucose, plasma insulin, C-peptide, and lipid parameters of pregnant women were estimated prior to delivery. Advanced serum glycation end-products (AGEs) were estimated at regular intervals in the heart and brain of pregnant rats. Monocyte Chemoattractant Protein-1 (MCP-1), NADPH oxidase 2 (Nox2), Role of advanced glycation end product (RAGE), Vascular endothelial growth factor (VEGF), p65, and vascular cell adhesion molecule 1 (VCAM-1) mRNA expression were estimated in the placenta. STZ-induced GDM pregnant rats showed significantly decreased placental weight and weight of fetal rats and dose-dependent ursolic acid treatment (p < .001) improved placental weight and weight of fetal rats at dose-dependent levels. After the ursolic acid treatment, serum blood glucose and lipid level were improved especially fasting blood glucose (FBG), high density lipoprotein (HDL), hepatic glycogen, fasting insulin (FINS), and serum insulin level and reached near to the normal control group rats. The antioxidant level of pancreas and liver were significantly (p < .001) reduced by the dose-dependent treatment of ursolic acid. Treatment with Ursolic acid moderately but not significantly decreases the risk of fetal development defects relative to the GDM group. The potential effect on fetal development in Pregnant Rats with Gestational Diabetes Mellitus via AGEs-RAGE signaling pathway was shown by Ursolic acid. PRACTICAL APPLICATIONS: As we know that the gestational diabetes mellitus increases worldwide day by day. In the current experimental study, we try to examine the gestational diabetic effect of ursolic acid. The finding of the current study showed the gestational diabetic protective effect in the female rats via AGEs-RAGE signaling pathway. The result showed the antioxidant, anti-inflammatory, and biochemical parameters. On the basis of the result, we can say that the ursolic acid can be the protective drug for treatment of gestational diabetes mellitus. CI - (c) 2021 Wiley Periodicals LLC. FAU - Dai, Senge AU - Dai S AD - Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, China. FAU - Meng, Xiaoyan AU - Meng X AD - Department of Obstetrics and Gynecology, Suzhou Wuzhong People's Hospital, Suzhou, China. FAU - Cai, Xiaqin AU - Cai X AD - Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, China. FAU - Yuan, Chun AU - Yuan C AD - Disinfection Supply Center, The First Hospital of HuBei WuHan, Wuhan, China. FAU - Zhao, Zhongmei AU - Zhao Z AD - Department of Gynecology, Yantai Municipal Laiyang Central Hospital, Laiyang, China. FAU - Zhong, Li AU - Zhong L AD - Department of Gynecology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. FAU - Shi, Yongmei AU - Shi Y AD - Department of Obstetrics and Gynecology, Tongzhou Maternal & Child Health Hospital of Beijing, Beijing, China. FAU - Yin, Fengling AU - Yin F AUID- ORCID: 0000-0002-8804-9874 AD - Department of Gynecology, Xuzhou Central Hospital, XuZhou Clinical School of Xuzhou Medical University, The Xuzhou School of Clinical Medicine of Nanjing Medical University, XuZhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Affiliated Hospital of Southeast University, Xuzhou, China. LA - eng PT - Journal Article DEP - 20210215 PL - United States TA - J Food Biochem JT - Journal of food biochemistry JID - 7706045 RN - 0 (Glycation End Products, Advanced) RN - 0 (Receptor for Advanced Glycation End Products) RN - 0 (Triterpenes) RN - 0 (Vascular Endothelial Growth Factor A) SB - IM MH - Animals MH - *Diabetes, Gestational/drug therapy MH - Female MH - Fetal Development MH - Glycation End Products, Advanced MH - Humans MH - Placenta MH - Pregnancy MH - Rats MH - Rats, Sprague-Dawley MH - Receptor for Advanced Glycation End Products MH - Signal Transduction MH - Triterpenes MH - Vascular Endothelial Growth Factor A MH - Ursolic Acid OTO - NOTNLM OT - AGE-RAGE signaling OT - fetal development OT - gestational diabetes mellitus OT - plasma insulin OT - ursolic acid EDAT- 2021/02/16 06:00 MHDA- 2021/07/09 06:00 CRDT- 2021/02/15 08:45 PHST- 2021/01/21 00:00 [revised] PHST- 2020/12/25 00:00 [received] PHST- 2021/01/28 00:00 [accepted] PHST- 2021/02/16 06:00 [pubmed] PHST- 2021/07/09 06:00 [medline] PHST- 2021/02/15 08:45 [entrez] AID - 10.1111/jfbc.13651 [doi] PST - ppublish SO - J Food Biochem. 2021 Apr;45(4):e13651. doi: 10.1111/jfbc.13651. Epub 2021 Feb 15.