PMID- 33588415
OWN - NLM
STAT- MEDLINE
DCOM- 20211223
LR  - 20211223
IS  - 1422-6421 (Electronic)
IS  - 1422-6405 (Linking)
VI  - 209
IP  - 4-6
DP  - 2020
TI  - Soluble Epoxide Hydrolase as an Important Player in Intestinal Cell 
      Differentiation.
PG  - 177-188
LID - 10.1159/000512807 [doi]
AB  - There is growing evidence that soluble epoxide hydrolase (sEH) may play a role in 
      cell differentiation. sEH metabolizes biologically highly active and generally 
      cytoprotective epoxyeicosatrienoic acids (EETs), generated from arachidonic acid 
      metabolism by CYP epoxygenases (CYP2C and CYP2J subfamilies), to less active 
      corresponding diols. We investigated the effect of sEH inhibitor (TPPU) on the 
      expression of villin, CYP2C8, CYP2C9, CYP2J2, and sEH in undifferentiated and in 
      vitro differentiated HT-29 and Caco2 cell lines. The administration of 10 muM TPPU 
      on differentiated HT-29 and Caco2 cells resulted in a significant decrease in 
      expression of villin, a marker for intestinal cell differentiation. It was 
      accompanied by a disruption of the brush border when microvilli appeared sparse 
      and short in atomic force microscope scans of HT-29 cells. Although inhibition of 
      sEH in differentiated HT-29 and Caco2 cells led to an increase in sEH expression 
      in both cell lines, this treatment had an opposite effect on CYP2J2 expression in 
      HT-29 and Caco2 cells. In addition, tissue samples of colorectal carcinoma and 
      adjacent normal tissues from 45 patients were immunostained for sEH and villin. 
      We detected a significant decrease in the expression of both proteins in 
      colorectal carcinoma in comparison to adjacent normal tissue, and the decrease in 
      both sEH and villin expression revealed a moderate positive association. Taken 
      together, our results showed that sEH is an important player in intestinal cell 
      differentiation.
CI  - (c) 2021 S. Karger AG, Basel.
FAU - Cizkova, Katerina
AU  - Cizkova K
AD  - Department of Histology and Embryology, Faculty of Medicine and Dentistry, 
      Palacky University, Olomouc, Czechia.
FAU - Koubova, Katerina
AU  - Koubova K
AD  - Department of Histology and Embryology, Faculty of Medicine and Dentistry, 
      Palacky University, Olomouc, Czechia.
FAU - Foltynkova, Tereza
AU  - Foltynkova T
AD  - Department of Histology and Embryology, Faculty of Medicine and Dentistry, 
      Palacky University, Olomouc, Czechia.
FAU - Jiravova, Jana
AU  - Jiravova J
AD  - Department of Medical Biophysics, Institute of Molecular and Translational 
      Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, 
      Czechia.
FAU - Tauber, Zdenek
AU  - Tauber Z
AD  - Department of Histology and Embryology, Faculty of Medicine and Dentistry, 
      Palacky University, Olomouc, Czechia, zdenek.tauber@upol.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210215
PL  - Switzerland
TA  - Cells Tissues Organs
JT  - Cells, tissues, organs
JID - 100883360
RN  - 0 (Eicosanoids)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP2J2)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
SB  - IM
MH  - Caco-2 Cells
MH  - *Cell Differentiation
MH  - Colorectal Neoplasms
MH  - Cytochrome P-450 CYP2J2
MH  - Cytochrome P-450 Enzyme System
MH  - Eicosanoids
MH  - Epoxide Hydrolases
MH  - Humans
OTO - NOTNLM
OT  - Arachidonic acid metabolism
OT  - Brush border
OT  - Cell differentiation models
OT  - Colorectal carcinoma
OT  - Soluble epoxide hydrolase inhibitor
EDAT- 2021/02/16 06:00
MHDA- 2021/12/24 06:00
CRDT- 2021/02/15 20:17
PHST- 2020/07/20 00:00 [received]
PHST- 2020/11/05 00:00 [accepted]
PHST- 2021/02/16 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/02/15 20:17 [entrez]
AID - 000512807 [pii]
AID - 10.1159/000512807 [doi]
PST - ppublish
SO  - Cells Tissues Organs. 2020;209(4-6):177-188. doi: 10.1159/000512807. Epub 2021 
      Feb 15.