PMID- 33588924 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210219 IS - 2045-3701 (Print) IS - 2045-3701 (Electronic) IS - 2045-3701 (Linking) VI - 11 IP - 1 DP - 2021 Feb 15 TI - Single-cell transcriptomic landscape reveals the differences in cell differentiation and immune microenvironment of papillary thyroid carcinoma between genders. PG - 39 LID - 10.1186/s13578-021-00549-w [doi] LID - 39 AB - BACKGROUND: Papillary thyroid carcinoma (PTC) is the main pathological type of thyroid carcinoma (TC). Gender is a prominent background parameter for patients with PTC. Here, we aimed to delineate the differences in cell clusters and immune microenvironment in relation to gender in PTC. METHODS: We generated 6720, 14,666, and 33,373 single-cell transcriptomes that were pooled from the tissues of four male patients with PTC, seven female patients with PTC, and three patients with nodular goiter, respectively. We performed single-cell RNA-sequencing (scRNA-seq) based on BD Rhapsody and characterized the first single-cell transcriptomic landscape of PTC involving gender. The differential cell clusters and their gene profiles were identified and analyzed via a multi-resolution network in male and female patients. The interactions of fibroblasts and endothelial cells with malignant epithelial cells and the difference in the immune infiltration of B and T lymphocytes according to gender were assessed. RESULTS: Malignant epithelial cells were divided into two distinct subsets in male and female patients with PTC. Moreover, significant differences involving inferred copy-number variations (CNVs), gene profiles, and cell differentiation were detected between male and female patients. Regarding the interactions of fibroblasts and endothelial cells with malignant epithelial cells, members of the human leukocyte antigen (HLA) family and their receptors were considered as typical in female patients with PTC, while transforming growth factor beta 1 (TGFB1) and its receptors were typical of male patients with PTC. The characteristics of B cells, including cell clusters, cell differentiation, and dominant gene sets, were significantly different between genders. CONCLUSIONS: Our data revealed the detailed differences in cell clusters and immune microenvironment in PTC according to gender at the single-cell level, which provided new insights into the understanding of the impact of gender on PTC. FAU - Peng, Miaoguan AU - Peng M AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Wei, Guohong AU - Wei G AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Zhang, Yunjian AU - Zhang Y AD - Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Li, Hai AU - Li H AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Lai, Yingrong AU - Lai Y AD - Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Guo, Yan AU - Guo Y AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Chen, Yuxin AU - Chen Y AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Liu, Liehua AU - Liu L AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Xiao, Haipeng AU - Xiao H AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. FAU - Guan, Hongyu AU - Guan H AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. ghongy@mail.sysu.edu.cn. FAU - Li, Yanbing AU - Li Y AD - Department of Endocrinology and Diabetes Center, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangdong, 510080, Guangzhou, China. liyb@mail.sysu.edu.cn. LA - eng GR - 2019B020230001/the Key Field Research and Development Program of Guangdong Province/ GR - 2018A030313599/Natural Science Foundation of Guangdong Province/ GR - 2019A1515012046/the Guangdong Basic and Applied Basic Research Foundation/ GR - 2020A1515010049/the Guangdong Basic and Applied Basic Research Foundation/ GR - 2018116212648808/Guangdong Medical Science and Technology Research Foundation/ GR - 20ykpy60/the Young Teachers Cultivate Projects of Sun Yat-sen University/ PT - Journal Article DEP - 20210215 PL - England TA - Cell Biosci JT - Cell & bioscience JID - 101561195 PMC - PMC7885238 OTO - NOTNLM OT - Cell differentiation OT - Gender-driven OT - Immune microenvironment OT - Papillary thyroid carcinoma OT - Single-cell RNA-sequencing COIS- The authors declare that they have no competing interests. EDAT- 2021/02/17 06:00 MHDA- 2021/02/17 06:01 PMCR- 2021/02/15 CRDT- 2021/02/16 05:44 PHST- 2020/12/03 00:00 [received] PHST- 2021/01/30 00:00 [accepted] PHST- 2021/02/16 05:44 [entrez] PHST- 2021/02/17 06:00 [pubmed] PHST- 2021/02/17 06:01 [medline] PHST- 2021/02/15 00:00 [pmc-release] AID - 10.1186/s13578-021-00549-w [pii] AID - 549 [pii] AID - 10.1186/s13578-021-00549-w [doi] PST - epublish SO - Cell Biosci. 2021 Feb 15;11(1):39. doi: 10.1186/s13578-021-00549-w.