PMID- 33592729
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20220531
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 225
DP  - 2021 Apr 1
TI  - Electrochemical detection of miRNA-100 in the sera of gastric cancer patients 
      based on DSN-assisted amplification.
PG  - 121981
LID - S0039-9140(20)31272-8 [pii]
LID - 10.1016/j.talanta.2020.121981 [doi]
AB  - Gastric cancer (GC) is a common malignant digestive tract tumor that leads to 
      high mortality worldwide. Early diagnosis of GC is very important for adequate 
      treatment. However, a rapid, specific and sensitive method for the detection of 
      GC is currently not available. Here, a biosensor CPs/AuNP-AuE, the gold 
      nanoparticle (AuNP)-modified Au electrode (AuE) which was coupled with DNA 
      capture probes (CPs), was developed to detect the content of miR-100 in the sera 
      of GC patients. The results showed that AuNPs were uniformly deposited on the 
      surface of AuE. AuNPs enhanced the electrical conductivity and improved the 
      effective area of AuE. CPs were successfully assembled on AuNP-AuE that could be 
      digested by duplex-specific nuclease (DSN) from the miR-100/CPs complex on the 
      electrode, improving the sensitivity of the biosensor by recycling miR-100. The 
      data revealed that the biosensor was highly specific for the detection of 
      miR-100, which had the ability to distinguish one base-pair mistake in miR-100. 
      The detection of the biosensor for miR-100 ranged from 100 aM to 10 pM and the 
      limit of detection (LOD) was estimated to be 100 aM. The detection results of 100 
      human sera samples using this biosensor indicated that the cutoff for the 
      detection of gastric cancer was 5 fM. Therefore the biosensor developed in our 
      study served as a rapid, specific and sensitive strategy for the detection of 
      gastric cancer in clinic.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Zhuang, Jianjian
AU  - Zhuang J
AD  - College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
FAU - Wan, Haitao
AU  - Wan H
AD  - College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
FAU - Zhang, Xiaobo
AU  - Zhang X
AD  - College of Life Sciences, Zhejiang University, Hangzhou, 310058, China. 
      Electronic address: zxb0812@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20201209
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (MIRN100 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - *Biosensing Techniques
MH  - Electrochemical Techniques
MH  - Gold
MH  - Humans
MH  - Limit of Detection
MH  - *Metal Nanoparticles
MH  - *MicroRNAs/blood/genetics
MH  - *Stomach Neoplasms/diagnosis/genetics
OTO - NOTNLM
OT  - Biosensor
OT  - Detection
OT  - Gastric cancer
OT  - Gold nanoparticle
OT  - miR-100
EDAT- 2021/02/18 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/02/17 01:00
PHST- 2020/08/03 00:00 [received]
PHST- 2020/11/25 00:00 [revised]
PHST- 2020/12/05 00:00 [accepted]
PHST- 2021/02/17 01:00 [entrez]
PHST- 2021/02/18 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0039-9140(20)31272-8 [pii]
AID - 10.1016/j.talanta.2020.121981 [doi]
PST - ppublish
SO  - Talanta. 2021 Apr 1;225:121981. doi: 10.1016/j.talanta.2020.121981. Epub 2020 Dec 
      9.