PMID- 33593142
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20211214
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 30
IP  - 7
DP  - 2021 Apr 1
TI  - The Chromatin Remodeling Complex CHD1 Regulates the Primitive State of 
      Mesenchymal Stromal Cells to Control Their Stem Cell Supporting Activity.
PG  - 363-373
LID - 10.1089/scd.2020.0166 [doi]
AB  - The primitive state (stemness) of mesenchymal stromal cells (MSCs) is responsible 
      for supporting the function of tissue-specific stem cells to regenerate damaged 
      tissues. However, molecular mechanisms regulating the stemness of MSCs remain 
      unknown. In this study, we found that the primitive state of MSCs is 
      hierarchically regulated by the expression levels of the chromatin remodeling 
      complex, CHD1, with CHD1 expression levels higher in the undifferentiated state, 
      and decreasing upon MSC differentiation. Consistently, CHD1 expression levels 
      decrease during progressive loss of clonogenic progenitors (CFU-F) induced by 
      passage cultures. Moreover, knockdown (KD) of CHD1 decreased CFU-F frequency, 
      whereas CHD1 overexpression increased it. In addition, the expression of stem 
      cell-specific genes was down- or upregulated upon KD or overexpression of CHD1, 
      respectively, accompanied by associated changes in chromatin condensation. 
      Importantly, altering CHD1 expression levels affected the ability of MSCs to 
      support the self-renewing expansion of hematopoietic stem cells (HSCs). 
      Furthermore, CHD1 levels were significantly decreased in MSCs from acute myeloid 
      leukemia or aplastic anemia patients, where CFU-F and HSC-supporting activities 
      are lost. Altogether, these findings show that chromatin remodeling by CHD1 is a 
      molecular parameter that influences the primitive state of MSCs and their stem 
      cell-supporting activity, which controls tissue regeneration.
FAU - Lee, Hae-Ri
AU  - Lee HR
AD  - Catholic High-Performance Cell Therapy Center, The Catholic University of Korea, 
      Seoul, Republic of Korea.
FAU - Yang, Seung-Jip
AU  - Yang SJ
AD  - Catholic High-Performance Cell Therapy Center, The Catholic University of Korea, 
      Seoul, Republic of Korea.
FAU - Choi, Hyun-Kyung
AU  - Choi HK
AD  - Catholic High-Performance Cell Therapy Center, The Catholic University of Korea, 
      Seoul, Republic of Korea.
FAU - Kim, Jin-A
AU  - Kim JA
AD  - Catholic High-Performance Cell Therapy Center, The Catholic University of Korea, 
      Seoul, Republic of Korea.
FAU - Oh, Il-Hoan
AU  - Oh IH
AD  - Catholic High-Performance Cell Therapy Center, The Catholic University of Korea, 
      Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (DNA-Binding Proteins)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (CHD1 protein, human)
SB  - IM
MH  - Adipogenesis/genetics
MH  - Cell Differentiation/*genetics
MH  - Cell Proliferation/genetics
MH  - Cells, Cultured
MH  - Chromatin Assembly and Disassembly/*genetics
MH  - Coculture Techniques
MH  - DNA Helicases/*genetics/metabolism
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Fetal Blood/cytology
MH  - *Gene Expression Regulation
MH  - Hematopoietic Stem Cells/cytology/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Osteogenesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - chromatin remodeling
OT  - mesenchymal stromal cells
OT  - stemness
EDAT- 2021/02/18 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/02/17 05:36
PHST- 2021/02/18 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/02/17 05:36 [entrez]
AID - 10.1089/scd.2020.0166 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2021 Apr 1;30(7):363-373. doi: 10.1089/scd.2020.0166.