PMID- 33596158
OWN - NLM
STAT- MEDLINE
DCOM- 20210527
LR  - 20221207
IS  - 1541-1087 (Electronic)
IS  - 0731-5724 (Linking)
VI  - 40
IP  - 4
DP  - 2021 May-Jun
TI  - l-Cysteine Stimulates the Effect of Vitamin D on Inhibition of Oxidative Stress, 
      IL-8, and MCP-1 Secretion in High Glucose Treated Monocytes.
PG  - 327-332
LID - 10.1080/07315724.2020.1850371 [doi]
AB  - Objective: Vitamin D deficiency is common in the general population and diabetic 
      patients, and supplementation with vitamin D is widely used to help lower 
      oxidative stress and inflammation. The cytokine storm in SARS-CoV2 infection has 
      been linked with both diabetes and Vitamin D deficiency. This study examined the 
      hypothesis that supplementation with vitamin D, in combination with l-cysteine 
      (LC), is better at reducing oxidative stress and thereby, more effective, at 
      inhibiting the secretion of the pro-inflammatory cytokines, Interleukin-8 (IL-8) 
      and monocyte chemoattractant protein-1 (MCP-1) in U937 monocytes exposed to high 
      glucose concentrations. Methods: U937 monocytes were pretreated with 1,25 (OH)(2) 
      vitamin D (VD, 10 nM) or LC (250 microM) or VD + LC for 24 h and then exposed to 
      control or high glucose (HG, 25 mM) for another 24 h. Results: There were 
      significantly greater reactive oxygen species (ROS) levels in monocytes treated 
      with HG than those in controls. Combined supplementation with VD and LC showed a 
      more significant reduction in ROS (46%) in comparison with treatment with LC 
      (19%) or VD (26%) alone in monocytes exposed to HG. Similarly, VD 
      supplementation, together with LC, caused a more significant inhibition in the 
      secretion of IL-8 (36% versus 16%) and MCP-1 (46% versus 26%) in comparison with 
      that of VD (10 nM) alone in high-glucose treated monocytes. Conclusions: These 
      results suggest that combined supplementation with vitamin D and LC has the 
      potential to be more effective than either VD or LC alone in lowering the risk of 
      oxidative stress and inflammation associated with type 2 diabetes or COVID-19 
      infection. Further, this combined vitamin D with LC/N-acetylcysteine may be a 
      potent alternative therapy for SARS-CoV2 infected subjects. This approach can 
      prevent cellular damage due to cytokine storm in comorbid systemic inflammatory 
      conditions, such as diabetes, obesity, and hypertension.
FAU - Jain, Sushil K
AU  - Jain SK
AD  - Department of Pediatrics and Center for Cardiovascular Diseases and Sciences, 
      Louisiana State University Health Sciences Center-Shreveport, Shreveport, 
      Louisiana, USA.
FAU - Micinski, David
AU  - Micinski D
AD  - Department of Pediatrics and Center for Cardiovascular Diseases and Sciences, 
      Louisiana State University Health Sciences Center-Shreveport, Shreveport, 
      Louisiana, USA.
FAU - Parsanathan, Rajesh
AU  - Parsanathan R
AUID- ORCID: 0000-0001-8973-3507
AD  - Department of Pediatrics and Center for Cardiovascular Diseases and Sciences, 
      Louisiana State University Health Sciences Center-Shreveport, Shreveport, 
      Louisiana, USA.
LA  - eng
GR  - R01 AT007442/AT/NCCIH NIH HHS/United States
GR  - R33 AT010637/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20210217
PL  - United States
TA  - J Am Coll Nutr
JT  - Journal of the American College of Nutrition
JID - 8215879
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 1406-16-2 (Vitamin D)
RN  - IY9XDZ35W2 (Glucose)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - COVID-19/immunology
MH  - Chemokine CCL2/metabolism
MH  - Cysteine/*administration & dosage
MH  - Diabetes Mellitus, Type 2/drug therapy
MH  - Dietary Supplements
MH  - Drug Therapy, Combination
MH  - Glucose/administration & dosage
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Monocytes/immunology/virology
MH  - Oxidative Stress/*drug effects
MH  - SARS-CoV-2/*immunology
MH  - U937 Cells
MH  - Vitamin D/*administration & dosage
MH  - Vitamin D Deficiency/drug therapy/immunology/virology
MH  - *COVID-19 Drug Treatment
OTO - NOTNLM
OT  - COVID-19
OT  - IL-8
OT  - MCP-1
OT  - Vitamin D
OT  - high glucose
OT  - inflammation
OT  - l-cysteine
EDAT- 2021/02/18 06:00
MHDA- 2021/05/28 06:00
CRDT- 2021/02/17 17:09
PHST- 2021/02/18 06:00 [pubmed]
PHST- 2021/05/28 06:00 [medline]
PHST- 2021/02/17 17:09 [entrez]
AID - 10.1080/07315724.2020.1850371 [doi]
PST - ppublish
SO  - J Am Coll Nutr. 2021 May-Jun;40(4):327-332. doi: 10.1080/07315724.2020.1850371. 
      Epub 2021 Feb 17.