PMID- 3359661
OWN - NLM
STAT- MEDLINE
DCOM- 19880531
LR  - 20190919
IS  - 0730-0077 (Print)
IS  - 0730-0077 (Linking)
VI  - 10
IP  - 3
DP  - 1988
TI  - Measurement of angiotensinogen in human serum by fluorescence polarization 
      immunoassay.
PG  - 485-503
AB  - Fluorescence polarization immunoassay (F.P.I.A.) has rarely been used to measure 
      components of the renin system. Using an Abbott TDX polarimeter and fluorescein - 
      labelled angiotensin I as a tracer we measured angiotensin I by F.P.I.A. 
      Combining this procedure with a renin incubation step enabled measurement of 
      angiotensinogen in human serum. Using sera from male patients and from pregnant 
      females, a good correlation between radioimmunoassay (R.I.A.) and F.P.I.A. was 
      found. Two procedures were developed; one involving taking samples from the renin 
      incubation mixture, the other involving measurement of generated angiotensin I at 
      intervals without interrupting the renin incubation procedure. F.P.I.A. is less 
      expensive and somewhat simpler than R.I.A. but, with the instrument used, it was 
      less sensitive. An unexpected observation was that human renin increases 
      polarization in solutions containing fluorescein labelled angiotensin I, 
      indicating possible binding of renin to angiotensin I.
FAU - Gordon, D B
AU  - Gordon DB
AD  - Veterans Administration Medical Center, Livermore, California 94550.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Clin Exp Hypertens A
JT  - Clinical and experimental hypertension. Part A, Theory and practice
JID - 8207790
RN  - 11002-13-4 (Angiotensinogen)
RN  - 9041-90-1 (Angiotensin I)
SB  - IM
MH  - Angiotensin I/*analysis/blood
MH  - Angiotensinogen/analysis/*blood
MH  - Female
MH  - *Fluorescence Polarization
MH  - Humans
MH  - Male
MH  - Pregnancy
MH  - *Radioimmunoassay
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
AID - 10.3109/10641968809033905 [doi]
PST - ppublish
SO  - Clin Exp Hypertens A. 1988;10(3):485-503. doi: 10.3109/10641968809033905.