PMID- 33597825
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210219
IS  - 1303-6092 (Electronic)
IS  - 1300-0152 (Print)
IS  - 1300-0152 (Linking)
VI  - 45
IP  - 1
DP  - 2021
TI  - Expression profiles of interferon-related genes in cells infected with influenza 
      A viruses or transiently transfected with plasmids encoding viral RNA polymerase.
PG  - 88-103
LID - 10.3906/biy-2005-73 [doi]
AB  - Influenza A viruses frequently change their genetic characteristics, which leads 
      to the emergence of new viruses. Consequently, elucidation of the relationship 
      between influenza A virus and host cells has a great importance to cope with 
      viral infections. In this study, it was aimed to determine expression profiles of 
      interferon response genes in human embryonic kidney 293 (HEK293) cells infected 
      with human (A/WSN-H1N1) and avian influenza A viruses 
      (duck/Pennsylvania/10218/84/H5N2) or transfected with plasmids encoding viral 
      RdRP subunits and, to obtain clues about the genes that may be important for the 
      viral pathogenesis. The HEK293 cells cultured in a 12-well plate were infected 
      with influenza A viruses or transfected with plasmids encoding viral polymerase. 
      Total RNA extraction and cDNA preparation were carried out with commercial kits. 
      Qiagen 96-well-RT(2) Profiler PCR Array plates designated for interferons 
      response genes were used for quantitation of the transcripts. The relative 
      quantities of transcripts were normalized with STAT3 gen, and the results were 
      evaluated. Quantitative RT-PCR results showed that there are substantial 
      differences of the interferon response gene transcription in cells infected with 
      viruses or transfected with plasmids. A higher number of interferon-related genes 
      were found to be downregulated in the cells infected with DkPen compared to WSN. 
      On the other hand, significant differences in the expression profiles of 
      interferon response genes were observed in the cells expressing viral PA protein. 
      In particular, avian influenza PA protein was found to cause more aggressive 
      changes on the transcript levels. Human and avian influenza A viruses cause a 
      substantial change in interferon response gene expression in HEK293 cells. 
      However, a higher number of genes were downregulated in the cells infected with 
      avian influenza DkPen compared to WSN. It has been also concluded that the viral 
      PA protein is one of the important viral factors affecting the transcript level 
      of host genes.
CI  - Copyright (c) 2021 The Author(s).
FAU - CaGlayan, Elif
AU  - CaGlayan E
AUID- ORCID: 0000-0002-4249-0824
AD  - Enstitute of Health Sciences, Marmara University, Istanbul Turkey.
FAU - Turan, Kadir
AU  - Turan K
AUID- ORCID: 0000-0003-4175-3423
AD  - Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Marmara 
      University, Istanbul Turkey.
LA  - eng
PT  - Journal Article
DEP - 20210209
PL  - Turkey
TA  - Turk J Biol
JT  - Turkish journal of biology = Turk biyoloji dergisi
JID - 9434434
PMC - PMC7877717
OTO - NOTNLM
OT  - Influenza A viruses
OT  - PCR array
OT  - influenza RdRP
OT  - interferon response
COIS- CONFLICT OF INTEREST: none declared
EDAT- 2021/02/19 06:00
MHDA- 2021/02/19 06:01
PMCR- 2021/02/09
CRDT- 2021/02/18 06:10
PHST- 2020/05/19 00:00 [received]
PHST- 2020/11/01 00:00 [accepted]
PHST- 2021/02/18 06:10 [entrez]
PHST- 2021/02/19 06:00 [pubmed]
PHST- 2021/02/19 06:01 [medline]
PHST- 2021/02/09 00:00 [pmc-release]
AID - 10.3906/biy-2005-73 [doi]
PST - epublish
SO  - Turk J Biol. 2021 Feb 9;45(1):88-103. doi: 10.3906/biy-2005-73. eCollection 2021.