PMID- 33598163
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2048-7177 (Print)
IS  - 2048-7177 (Electronic)
IS  - 2048-7177 (Linking)
VI  - 9
IP  - 2
DP  - 2021 Feb
TI  - Influence of germinated brown rice-based flour modified by MAse on type 2 
      diabetic mice and HepG2 cell cytotoxic capacity.
PG  - 781-793
LID - 10.1002/fsn3.2043 [doi]
AB  - This study aimed to discover whether using maltogenic amylase (MAse) to modify 
      starch in germinated brown rice flour may enhance slow digestion starch and 
      release more bioactive compounds (BCs) content. To achieve this aim, the starch 
      was modified with four levels of MAse (0 U, 133 U, 266 U and 399 U MAse/g flour) 
      for 1 hr at pH 5 and then spray-dried to make modified flour. The biochemical 
      impacts of the products were then accessed in normal and type 2 diabetic mice for 
      4 weeks. The result showed that when the starch was modified by MAse 266 U/g, a 
      significant reduction of rapidly digested starch to 22.35% from 61.56%, an 
      increase in slowly digested starch to 33.09% while resistant starch as 2.92% 
      corresponding to the increase of gamma-amino butyric acid to 528.1 +/- 44.1 mg/L and 
      120.6 +/- 10.9 mg/L of ferulic acid. The extract from modified flour showed very 
      strong cytotoxic activity against HepG2 cell (>80% inhibition). The result in 
      vivo showed that the type-2 diabetic mice fed with this modified product could 
      better improve the stability of the glycemic index. Also, atherosclerotic plaque 
      assessment further supports these findings. The results indicated that BCs 
      released considerably couple with the changes in starch properties caused by MAse 
      enhanced the effectiveness of this product to diabetes as well as positive effect 
      on cytotoxic activity against HepG2 cell.
CI  - (c) 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.
FAU - Nguyen, Ngoc Thi Le
AU  - Nguyen NTL
AD  - Food Technology Department College of Agriculture Can Tho City Vietnam.
FAU - Nguyen, Binh Duong Thanh
AU  - Nguyen BDT
AD  - Food Technology Department College of Agriculture Can Tho City Vietnam.
FAU - Dai, Trang Thi Xuan
AU  - Dai TTX
AD  - Department of Biology College of Natural Science Can Tho City Vietnam.
FAU - Co, Son Hong
AU  - Co SH
AD  - National Agro - Forestry - Fishery Quality Assurance Department Can Tho City 
      Vietnam.
FAU - Do, Thao Thi
AU  - Do TT
AD  - Institute of Biotechnology Vietnam Academy of Science and Technology (VAST) Hanoi 
      Vietnam.
FAU - Tong Thi, Anh Ngoc
AU  - Tong Thi AN
AUID- ORCID: 0000-0002-5163-7621
AD  - Food Technology Department College of Agriculture Can Tho City Vietnam.
FAU - Oladapo, Ibitoye Joshua
AU  - Oladapo IJ
AD  - Food Technology Department College of Agriculture Can Tho City Vietnam.
FAU - Nguyen Cong, Ha
AU  - Nguyen Cong H
AUID- ORCID: 0000-0001-9921-9288
AD  - Food Technology Department College of Agriculture Can Tho City Vietnam.
LA  - eng
PT  - Journal Article
DEP - 20201129
PL  - United States
TA  - Food Sci Nutr
JT  - Food science & nutrition
JID - 101605473
PMC - PMC7866618
OTO - NOTNLM
OT  - bioactive compounds
OT  - cytotoxic activity
OT  - germinated brown rice
OT  - maltogenic amylase
OT  - modified starch
OT  - starch digestion
EDAT- 2021/02/19 06:00
MHDA- 2021/02/19 06:01
PMCR- 2020/11/29
CRDT- 2021/02/18 06:11
PHST- 2020/07/08 00:00 [received]
PHST- 2020/11/14 00:00 [revised]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2021/02/18 06:11 [entrez]
PHST- 2021/02/19 06:00 [pubmed]
PHST- 2021/02/19 06:01 [medline]
PHST- 2020/11/29 00:00 [pmc-release]
AID - FSN32043 [pii]
AID - 10.1002/fsn3.2043 [doi]
PST - epublish
SO  - Food Sci Nutr. 2020 Nov 29;9(2):781-793. doi: 10.1002/fsn3.2043. eCollection 2021 
      Feb.