PMID- 33598643
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210219
IS  - 2589-0042 (Electronic)
IS  - 2589-0042 (Linking)
VI  - 24
IP  - 2
DP  - 2021 Feb 19
TI  - Engineering living building materials for enhanced bacterial viability and 
      mechanical properties.
PG  - 102083
LID - 10.1016/j.isci.2021.102083 [doi]
LID - 102083
AB  - Living building materials (LBMs) utilize microorganisms to produce construction 
      materials that exhibit mechanical and biological properties. A hydrogel-based LBM 
      containing bacteria capable of microbially induced calcium carbonate 
      precipitation (MICP) was recently developed. Here, LBM design factors, i.e., 
      gel/sand ratio, inclusion of trehalose, and MICP pathways, are evaluated. The 
      results show that non-saturated LBM (gel/sand = 0.13) and gel-saturated LBM 
      (gel/sand = 0.30) underwent distinct failure modes. The inclusion of trehalose 
      maintains bacterial viability under ambient conditions with low relative 
      humidity, without affecting mechanical properties of the LBM. Comparison of 
      biotic and abiotic LBM shows that MICP efficiency in this material is subject to 
      the pathway selected: the LBM with heterotrophic ureolytic Escherichia coli 
      demonstrated the most mechanical enhancement from the abiotic controls, compared 
      with either ureolytic or CO(2)-concentrating mechanisms from Synechococcus. The 
      study shows that tailoring of LBM properties can be accomplished in a manner that 
      considers both LBM microstructure and MICP pathways.
CI  - (c) 2021 The Author(s).
FAU - Qiu, Jishen
AU  - Qiu J
AD  - Department of Civil, Environmental, and Architectural Engineering, University of 
      Colorado Boulder, 1111 Engineering Dr, Boulder, CO 80309, USA.
FAU - Cook, Sherri
AU  - Cook S
AD  - Department of Civil, Environmental, and Architectural Engineering, University of 
      Colorado Boulder, 1111 Engineering Dr, Boulder, CO 80309, USA.
FAU - Srubar, Wil V 3rd
AU  - Srubar WV 3rd
AD  - Department of Civil, Environmental, and Architectural Engineering, University of 
      Colorado Boulder, 1111 Engineering Dr, Boulder, CO 80309, USA.
FAU - Hubler, Mija H
AU  - Hubler MH
AD  - Department of Civil, Environmental, and Architectural Engineering, University of 
      Colorado Boulder, 1111 Engineering Dr, Boulder, CO 80309, USA.
FAU - Artier, Juliana
AU  - Artier J
AD  - Renewable and Sustainable Energy Institute, University of Colorado Boulder, 4001 
      Discovery Dr, Boulder, CO 80303, USA.
FAU - Cameron, Jeffrey C
AU  - Cameron JC
AD  - Renewable and Sustainable Energy Institute, University of Colorado Boulder, 4001 
      Discovery Dr, Boulder, CO 80303, USA.
LA  - eng
PT  - Journal Article
DEP - 20210121
PL  - United States
TA  - iScience
JT  - iScience
JID - 101724038
PMC - PMC7868992
OTO - NOTNLM
OT  - Biomaterials
OT  - Civil Engineering
OT  - Composite Materials
OT  - Materials Synthesis
COIS- W.V.S., S.C., M.H.H., and J.C.C. are inventors on a patent application filed by 
      the University of Colorado on the basis of this work (patent application no. 
      PCT/US2020/020863; filed on 3 April 2020). The authors declare they have no other 
      competing interests.
EDAT- 2021/02/19 06:00
MHDA- 2021/02/19 06:01
PMCR- 2021/01/21
CRDT- 2021/02/18 06:13
PHST- 2020/11/03 00:00 [received]
PHST- 2020/12/18 00:00 [revised]
PHST- 2021/01/14 00:00 [accepted]
PHST- 2021/02/18 06:13 [entrez]
PHST- 2021/02/19 06:00 [pubmed]
PHST- 2021/02/19 06:01 [medline]
PHST- 2021/01/21 00:00 [pmc-release]
AID - S2589-0042(21)00051-1 [pii]
AID - 102083 [pii]
AID - 10.1016/j.isci.2021.102083 [doi]
PST - epublish
SO  - iScience. 2021 Jan 21;24(2):102083. doi: 10.1016/j.isci.2021.102083. eCollection 
      2021 Feb 19.