PMID- 33600162
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 55
IP  - 5
DP  - 2021 Mar 2
TI  - Long-Term Fertilization Shapes the Putative Electrotrophic Microbial Community in 
      Paddy Soils Revealed by Microbial Electrosynthesis Systems.
PG  - 3430-3441
LID - 10.1021/acs.est.0c08022 [doi]
AB  - Electrotrophs play an important role in biogeochemical cycles, but the effects of 
      long-term fertilization on electrotrophic communities in paddy soils remain 
      unclear. Here, we explored the responses of electrotrophic communities in paddy 
      soil-based microcosms to different long-term fertilization practices using 
      microbial electrosynthesis systems (MESs), high-throughput quantitative PCR, and 
      16s rRNA gene-based Illumina sequencing techniques. Compared to the case in the 
      unfertilized soil (CK), applications of only manure (M); only chemical nitrogen, 
      phosphorous, and potassium fertilizers (NPK); and M plus NPK (MNPK) clearly 
      changed the electrotrophic bacterial community structure. The Streptomyces genus 
      of the Actinobacteria phylum was the dominant electrotroph in the CK, M, and MNPK 
      soils. The latter two soils also favored Truepera of Deinococcus-Thermus or 
      Arenimonas and Thioalkalispira of Proteobacteria. Furthermore, Pseudomonas of 
      Proteobacteria and Bacillus of Firmicutes were major electrotrophs in the NPK 
      soil. These electrotrophs consumed biocathodic currents coupled with nitrate 
      reduction and recovered 18-38% of electrons via dissimilatory nitrate reduction 
      to ammonium (DNRA). The increased abundances of the nrfA gene for DNRA induced by 
      electrical potential further supported that the electrotrophs enhanced DNRA for 
      all soils. These expand our knowledge about the diversity of electrotrophs and 
      their roles in N cycle in paddy soils and highlight the importance of 
      fertilization in shaping electrotrophic communities.
FAU - Li, Xiao-Min
AU  - Li XM
AD  - State Key Laboratory of Urban and Regional Ecology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road, No. 18, 
      Haidian District, Beijing 100085, China.
AD  - Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese 
      Academy of Sciences, Jimei Road, No. 1799, Jimei District, Xiamen 361021, China.
AD  - Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, 
      Ningbo Urban Environment Observation and Research Station, Chinese Academy of 
      Sciences, Zhongke Road 88, Beilun District, Ningbo 315830, China.
AD  - University of Chinese Academy of Sciences, Yuquan Road, No. 19A, Shijingshan 
      District, Beijing 100049, China.
FAU - Ding, Long-Jun
AU  - Ding LJ
AUID- ORCID: 0000-0002-8384-8351
AD  - State Key Laboratory of Urban and Regional Ecology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road, No. 18, 
      Haidian District, Beijing 100085, China.
FAU - Zhu, Dong
AU  - Zhu D
AUID- ORCID: 0000-0002-0826-6423
AD  - State Key Laboratory of Urban and Regional Ecology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road, No. 18, 
      Haidian District, Beijing 100085, China.
FAU - Zhu, Yong-Guan
AU  - Zhu YG
AUID- ORCID: 0000-0003-3861-8482
AD  - State Key Laboratory of Urban and Regional Ecology, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road, No. 18, 
      Haidian District, Beijing 100085, China.
AD  - Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese 
      Academy of Sciences, Jimei Road, No. 1799, Jimei District, Xiamen 361021, China.
AD  - University of Chinese Academy of Sciences, Yuquan Road, No. 19A, Shijingshan 
      District, Beijing 100049, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210218
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Fertilizers)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Fertilization
MH  - Fertilizers/analysis
MH  - *Microbiota
MH  - Nitrogen/analysis
MH  - *Oryza
MH  - RNA, Ribosomal, 16S/genetics
MH  - Soil
MH  - Soil Microbiology
EDAT- 2021/02/19 06:00
MHDA- 2021/04/24 06:00
CRDT- 2021/02/18 17:11
PHST- 2021/02/19 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2021/02/18 17:11 [entrez]
AID - 10.1021/acs.est.0c08022 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2021 Mar 2;55(5):3430-3441. doi: 10.1021/acs.est.0c08022. 
      Epub 2021 Feb 18.