PMID- 33603840
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210220
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 3
DP  - 2021 Mar
TI  - Long non-coding RNA MIAT promotes the proliferation and invasion of laryngeal 
      squamous cell carcinoma cells by sponging microRNA-613.
PG  - 232
LID - 10.3892/etm.2021.9663 [doi]
LID - 232
AB  - Accumulating evidence indicates that the long non-coding RNA myocardial 
      infarction associated transcript (lncRNA MIAT) serves an important role in the 
      progression of a number of cancer types. However, the precise molecular mechanism 
      of MIAT in laryngeal squamous cell carcinoma (LSCC) progression remain elusive. 
      The aim of the current study was to assess the effects and to clarify the 
      molecular mechanism of MIAT on the proliferation and invasion of LSCC cells. The 
      expression of MIAT was detected in LSCC tissues and cells using reverse 
      transcription-quantitative PCR. MTT and colony formation assays were performed to 
      examine the effects of MIAT on the proliferation of LSCC cells. Additionally, 
      wound healing and Transwell experiments were employed to examine cellular 
      migration and invasion. Luciferase reporter gene assay was also used to confirm 
      the direct binding between MIAT and microRNA (miR)-613 in LSCC cells. An RNA 
      immunoprecipitation assay was performed to verify the interaction between MIAT 
      and miR-613. In the present study, it was found that the expression of MIAT in 
      LSCC tissues was markedly higher compared with that in adjacent non-tumor 
      tissues. In addition, MIAT expression was also increased in the human LSCC cell 
      lines TU686, TU-177 and AMC-HN-8 compared with that in normal human keratinocytes 
      (HaCaT). Knocking down MIAT expression significantly reduced LSCC cell 
      proliferation and inhibited colony formation, a shown by MTT and colony formation 
      assays, respectively. MIAT knockdown also substantially inhibited the migratory 
      and invasive abilities of LSCC cells, as shown by wound healing and Transwell 
      invasion assays, respectively. Subsequently, luciferase reporter assays verified 
      that MIAT could bind to miR-613, where a negative correlation was observed 
      between the expression of MIAT and miR-613 in LSCC tissues. Suppression of 
      miR-613 partially reversed the inhibitory effects of MIAT knockdown on the 
      proliferation, migration and invasion of LSCC cells. Taken together, the present 
      study identified that MIAT may function as an oncogenic lncRNA to promote LSCC 
      progression, which provides a potential therapeutic target or as a novel 
      diagnostic biomarker for LSCC.
CI  - Copyright: (c) Song et al.
FAU - Song, Fucun
AU  - Song F
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medical Center, 
      Tianjin 300121, P.R. China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medical Center, 
      Tianjin 300121, P.R. China.
FAU - Liu, Jixiang
AU  - Liu J
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medical Center, 
      Tianjin 300121, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210121
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7851618
OTO - NOTNLM
OT  - invasion
OT  - laryngeal squamous cell carcinoma
OT  - long non-coding RNA
OT  - myocardial infarction associated transcript
OT  - proliferation
EDAT- 2021/02/20 06:00
MHDA- 2021/02/20 06:01
PMCR- 2021/01/21
CRDT- 2021/02/19 06:06
PHST- 2019/11/22 00:00 [received]
PHST- 2020/11/24 00:00 [accepted]
PHST- 2021/02/19 06:06 [entrez]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2021/02/20 06:01 [medline]
PHST- 2021/01/21 00:00 [pmc-release]
AID - ETM-0-0-09663 [pii]
AID - 10.3892/etm.2021.9663 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Mar;21(3):232. doi: 10.3892/etm.2021.9663. Epub 2021 Jan 21.