PMID- 33604504
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220420
IS  - 2514-1775 (Electronic)
IS  - 2514-1775 (Linking)
VI  - 5
IP  - 1
DP  - 2021
TI  - Exploring molecular pathology of chronic kidney disease in systemic sclerosis by 
      analysis of urinary and serum proteins.
PG  - rkaa083
LID - 10.1093/rap/rkaa083 [doi]
LID - rkaa083
AB  - OBJECTIVE: Renal involvement is common in systemic sclerosis (scleroderma; SSc) 
      and includes chronic kidney disease (CKD). We have performed analysis of urinary 
      proteins to gain insight into local molecular pathology of CKD in SSc and 
      identify candidate markers for use in clinical trials. METHODS: To evaluate 
      urinary proteins that might specifically reflect SSc-related CKD, patients were 
      recruited with confirmed SSc and stratified for the presence or absence of CKD. 
      Controls included patients with CKD and no SSc, in addition to healthy 
      volunteers. Candidate markers were measured in serum and urine by multiplex 
      immunoassay testing for IL6, IL18, TNF-alpha, monocyte chemoattractant protein 1 
      (MCP1), monocyte chemoattractant protein 3 (MCP3), VEGF and the soluble adhesion 
      molecules vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion 
      molecule 1 (ICAM-1). RESULTS: One hundred and two subjects were examined, 
      including patients with SSc with no evidence of CKD (n = 40), SSc with CKD 
      (n = 39), non-SSc CKD (n = 11) and healthy volunteers (n = 12). Urinary levels of 
      IL6, MCP1, TNF-alpha, MCP3, IL18 and ICAM-1 were elevated in SSc patients compared 
      with healthy controls. The most significant differences were for MCP1 and ICAM-1 
      (both P < 0.0001), and these analytes also showed the most significant 
      differences between groups overall (P = 0.003 for MCP1 and P < 0.0001 for 
      ICAM-1). These markers showed a trend (MCP1, P = 0.0868) or a significant 
      difference (ICAM-1, P = 0.0134) between SSc-CKD and SSc with normal renal 
      function. CONCLUSION: Urinary levels of candidate molecular markers appear to 
      reflect SSc-CKD more than serum markers. MCP1 and ICAM-1 are promising molecular 
      markers for SSc-CKD and might be potential biomarkers of SSc renal involvement. 
      This might be explored in future prospective analyses.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of the 
      British Society for Rheumatology.
FAU - Stern, Edward P
AU  - Stern EP
AD  - UCL Centre for Rheumatology and Connective Tissue Diseases.
FAU - Unwin, Robert
AU  - Unwin R
AD  - UCL Department of Renal Medicine, UCL, London.
AD  - AstraZeneca BioPharmaceuticals R&D, Early CVRM, Cambridge, UK.
FAU - Burns, Aine
AU  - Burns A
AD  - UCL Department of Renal Medicine, UCL, London.
FAU - Ong, Voon H
AU  - Ong VH
AUID- ORCID: 0000-0001-5474-0053
AD  - UCL Centre for Rheumatology and Connective Tissue Diseases.
FAU - Denton, Christopher P
AU  - Denton CP
AUID- ORCID: 0000-0003-3975-8938
AD  - UCL Centre for Rheumatology and Connective Tissue Diseases.
LA  - eng
GR  - MR/K015230/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20210107
PL  - England
TA  - Rheumatol Adv Pract
JT  - Rheumatology advances in practice
JID - 101736676
PMC - PMC7878848
OTO - NOTNLM
OT  - adhesion molecule
OT  - biomarker
OT  - chemokine
OT  - renal
OT  - scleroderma
OT  - systemic sclerosis
EDAT- 2021/02/20 06:00
MHDA- 2021/02/20 06:01
PMCR- 2021/01/07
CRDT- 2021/02/19 06:08
PHST- 2020/11/09 00:00 [received]
PHST- 2020/12/11 00:00 [revised]
PHST- 2021/02/19 06:08 [entrez]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2021/02/20 06:01 [medline]
PHST- 2021/01/07 00:00 [pmc-release]
AID - rkaa083 [pii]
AID - 10.1093/rap/rkaa083 [doi]
PST - epublish
SO  - Rheumatol Adv Pract. 2021 Jan 7;5(1):rkaa083. doi: 10.1093/rap/rkaa083. 
      eCollection 2021.