PMID- 33605427
OWN - NLM
STAT- MEDLINE
DCOM- 20210830
LR  - 20210830
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Linking)
VI  - 59
IP  - 1
DP  - 2021
TI  - Genistein exerts a cell-protective effect via Nrf2/HO-1/ /PI3K signaling in 
      Ab25-35-induced Alzheimer's disease models in vitro.
PG  - 49-56
LID - 10.5603/FHC.a2021.0006 [doi]
AB  - INTRODUCTION: Alzheimer's disease (AD), a very common neurodegenerative disorder, 
      is mainly characterized by the deposition of b-amyloid protein (Ab) and extensive 
      neuronal cell death. Currently, there are no satisfactory therapeutic approaches 
      for AD. Although neuroprotective effects of genistein against Ab-induced toxicity 
      have been reported, the underlying molecular mechanisms remain unclear. 
      Furthermore, the PI3K/Akt/Nrf2 signaling pathway is associated with AD. The aim 
      of the study was to investigate whether genistein can modulate Nrf2/HO-1/PI3K 
      signaling to treat AD. MATERIALS AND METHODS: Cell viability assay, the 
      measurement of heme oxygenase-1 (HO-1) expression by reverse 
      transcription-polymerase chain reaction (RT-qPCR), and western blot were 
      performed on the SH-SY5Y cells induced by Ab25-35 in response to the treatment 
      with genistein. Moreover, PI3K p85 phosphorylation was measured. RESULTS: 
      Genistein enhanced the HO-1expression at both the mRNA and protein levels, as 
      well as the PI3K p85 phosphorylation level. In addition, genistein increased the 
      survival of SH-SY5Y cells treated with Ab25-35via HO-1 signaling. However, 
      following transfection with Nrf2 small interfering RNA (siRNA) and treatment with 
      LY294002, an inhibitor of PI3K p85, genistein could not upregulate HO-1 to exert 
      neuroprotective effects on SH-SY5Y cells treated with Ab25-35. CONCLUSIONS: These 
      results suggest that genistein exerts a neuroprotective effect on SH-SY5Y cells 
      in vitro via Nrf2/ HO-1/PI3K signaling, providing a foundation for the 
      application of genistein in the treatment of neurodegenerative diseases related 
      to Nrf2/HO-1/PI3K signaling.
FAU - Yi, Shanqing
AU  - Yi S
AD  - The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, 
      PR China.
FAU - Chen, Shuangxi
AU  - Chen S
AD  - The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, 
      PR China.
FAU - Xiang, Jian
AU  - Xiang J
AD  - Second People's Hospital of Shaoyang City, Shaoyang, Hunan 422001, PR China.
FAU - Tan, Jian
AU  - Tan J
AD  - The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, 
      PR China.
FAU - Huang, Kailiang
AU  - Huang K
AD  - The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, 
      PR China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 
      421001, PR China.
FAU - Wang, Yilin
AU  - Wang Y
AD  - The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, 
      PR China.
AD  - The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 
      421001, PR China.
FAU - Wu, Heng
AU  - Wu H
AD  - The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, 
      PR China. c137001106@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210219
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (25-35))
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.1.- (PIK3R1 protein, human)
RN  - EC 2.7.1.137 (Class Ia Phosphatidylinositol 3-Kinase)
SB  - IM
MH  - Alzheimer Disease/chemically induced/*drug therapy
MH  - Amyloid beta-Peptides
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Class Ia Phosphatidylinositol 3-Kinase/metabolism
MH  - Genistein/*pharmacology
MH  - Humans
MH  - NF-E2-Related Factor 2/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Peptide Fragments
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Nrf2
OT  - Nrf2/HO-1/PI3K pathway
OT  - SH-SY5Y cells
OT  - amyloid b25-35
OT  - genistein
OT  - heme oxygenase-1
OT  - in vitro
OT  - siRNA
EDAT- 2021/02/20 06:00
MHDA- 2021/08/31 06:00
CRDT- 2021/02/19 08:40
PHST- 2020/12/17 00:00 [received]
PHST- 2021/02/09 00:00 [accepted]
PHST- 2021/02/04 00:00 [revised]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2021/08/31 06:00 [medline]
PHST- 2021/02/19 08:40 [entrez]
AID - VM/OJS/J/72393 [pii]
AID - 10.5603/FHC.a2021.0006 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2021;59(1):49-56. doi: 10.5603/FHC.a2021.0006. Epub 
      2021 Feb 19.