PMID- 33606108
OWN - NLM
STAT- MEDLINE
DCOM- 20211206
LR  - 20211214
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 44
IP  - 6
DP  - 2021 Jun
TI  - Bioengineered gold nanoparticles using Cynodon dactylon extract and its 
      cytotoxicity and antibacterial activities.
PG  - 1253-1262
LID - 10.1007/s00449-021-02527-5 [doi]
AB  - In this study, simple and green route approach was applied for the synthesis gold 
      nanoparticles (AuNPs) containing an aqueous extract of Cynodon dactylon L. Pers., 
      (C. dactylon). The synthesized AuNPs were characterized using spectral and 
      microscopic analysis. The changes in the color pattern were observed upon 
      synthesis by UV-vis spectrophotometer with a peak of 530 nm. The FT-IR, XRD, SEM, 
      and TEM were used to analyze the crystal nature and morphology of the green 
      synthesized AuNPs. The C. dactylon-loaded AuNPs in different concentrations 
      (0.625-100 mug/ml) were used to assess cytotoxicity activity against MCF-7 cell 
      line and where the IC(50) was found to be 31.34 mug/ml by MTT assay. The C. 
      dactylon-AuNPs were significantly increased reactive oxygen species (ROS) 
      generation, DNA fragmentation, and mitochondrial membrane changes observed by 
      dichlorodihydroflurescenin diacetate (DCFH-DA), 4',6-diamidino-2-phenylindole 
      (DAPI), Rhodamine-123, and acridine orange (AO)/ethidium bromide (EtBr) staining 
      assay. Besides the microbial study revealed that C. dactylon-AuNPs exhibited 
      significant antibacterial activity against clinically isolated pathogenic 
      bacteria such as Enterobacter cloacae, Staphylococus Haemolytics, Staphylococcus 
      petrasii subsp. Pragensis and Bacillus cereus with a zone of inhibition 13, 12, 
      13 and 12 mm, respectively. It could be concluded that C. dactylon has the 
      ability to be involved in the biosynthesis of AuNPs, and the pharmacological 
      studies proved the promising cytotoxic effect on MCF-7 cell line and pathogenic 
      bacterial species.
FAU - Vinayagam, Ramachandran
AU  - Vinayagam R
AD  - Department of Biotechnology, Institute of Biotechnology, College of Life and 
      Applied Sciences, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk, 
      38541, Republic of Korea.
FAU - Santhoshkumar, Murali
AU  - Santhoshkumar M
AD  - Department of Biotechnology, Thiruvalluvar University, Serkadu, Vellore, Tamil 
      Nadu, 632 115, India.
FAU - Lee, Kyung Eun
AU  - Lee KE
AD  - Department of Biotechnology, Institute of Biotechnology, College of Life and 
      Applied Sciences, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk, 
      38541, Republic of Korea.
AD  - Stemforce, 313 Institute of Industrial Technology, Yeungnam University, 280 
      Daehak-Ro, Gyeongsan, Gyeongbuk, 38541, Republic of Korea.
FAU - David, Ernest
AU  - David E
AD  - Department of Biotechnology, Thiruvalluvar University, Serkadu, Vellore, Tamil 
      Nadu, 632 115, India.
FAU - Kang, Sang Gu
AU  - Kang SG
AUID- ORCID: 0000-0002-5216-5494
AD  - Department of Biotechnology, Institute of Biotechnology, College of Life and 
      Applied Sciences, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk, 
      38541, Republic of Korea. kangsg@ynu.ac.kr.
LA  - eng
GR  - 2020-D-G043-010119/LINC+ (Leaders in INdustry-university Cooperation +/
PT  - Journal Article
DEP - 20210219
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cytotoxins)
RN  - 0 (Plant Extracts)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - *Anti-Bacterial Agents/chemical synthesis/chemistry/pharmacology
MH  - Bacteria/*growth & development
MH  - Bioengineering
MH  - Cynodon/*chemistry
MH  - *Cytotoxins/chemical synthesis/chemistry/pharmacology
MH  - *Gold/chemistry/pharmacology
MH  - Humans
MH  - MCF-7 Cells
MH  - *Metal Nanoparticles/chemistry/therapeutic use
MH  - Plant Extracts/*chemistry
OTO - NOTNLM
OT  - Antimicrobial
OT  - Cynodon dactylon L.
OT  - Cytotoxicity
OT  - Gold nanoparticles
OT  - Reactive oxygen species
EDAT- 2021/02/20 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/02/19 12:13
PHST- 2020/11/03 00:00 [received]
PHST- 2021/01/31 00:00 [accepted]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/02/19 12:13 [entrez]
AID - 10.1007/s00449-021-02527-5 [pii]
AID - 10.1007/s00449-021-02527-5 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2021 Jun;44(6):1253-1262. doi: 
      10.1007/s00449-021-02527-5. Epub 2021 Feb 19.