PMID- 33606846
OWN - NLM
STAT- MEDLINE
DCOM- 20210823
LR  - 20210823
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 2
DP  - 2021
TI  - Visualization of endogenous gut bacteria in Drosophila melanogaster using 
      fluorescence in situ hybridization.
PG  - e0247376
LID - 10.1371/journal.pone.0247376 [doi]
LID - e0247376
AB  - All metazoans are colonized by a complex and diverse set of microorganisms. The 
      microbes colonize all parts of the body and are especially abundant in the 
      gastrointestinal tract, where they constitute the gut microbiome. The fruit fly 
      Drosophila melanogaster turned out to be an exquisite model organism to 
      functionally test the importance of an intact gut microbiome. Still, however, 
      fundamental questions remain unanswered. For example, it is unknown whether a 
      fine-tuned regionalization of the gut microbiome exists and how such a spatial 
      organization could be established. In order to pave the way for answering this 
      question, we generated an optimized and adapted fluorescence in situ 
      hybridization (FISH) protocol. We focused on the detection of the two major 
      Drosophila gut microbiome constituting bacteria genera: Acetobacter and 
      Lactobacillus. FISH allows to detect the bacteria in situ and thus to investigate 
      their spatial localization in respect to the host as well as to other microbiome 
      members. We demonstrate the applicability of the protocol using a diverse set of 
      sample types.
FAU - Akhtar, Irfan
AU  - Akhtar I
AD  - Institut fur Mathematische Modellierung Biologischer Systeme, 
      Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany.
AD  - Systembiologie des Fettstoffwechsels, Heinrich-Heine-Universitat Dusseldorf, 
      Dusseldorf, Germany.
FAU - Stewart, Fiona A
AU  - Stewart FA
AD  - Institut fur Mathematische Modellierung Biologischer Systeme, 
      Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany.
AD  - Systembiologie des Fettstoffwechsels, Heinrich-Heine-Universitat Dusseldorf, 
      Dusseldorf, Germany.
FAU - Harle, Anna
AU  - Harle A
AD  - Institut fur Mathematische Modellierung Biologischer Systeme, 
      Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany.
AD  - Systembiologie des Fettstoffwechsels, Heinrich-Heine-Universitat Dusseldorf, 
      Dusseldorf, Germany.
FAU - Droste, Andrea
AU  - Droste A
AD  - Institut fur Mathematische Modellierung Biologischer Systeme, 
      Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany.
AD  - Systembiologie des Fettstoffwechsels, Heinrich-Heine-Universitat Dusseldorf, 
      Dusseldorf, Germany.
FAU - Beller, Mathias
AU  - Beller M
AUID- ORCID: 0000-0003-0987-0080
AD  - Institut fur Mathematische Modellierung Biologischer Systeme, 
      Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany.
AD  - Systembiologie des Fettstoffwechsels, Heinrich-Heine-Universitat Dusseldorf, 
      Dusseldorf, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210219
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Acetobacter/genetics/isolation & purification
MH  - Animals
MH  - Bacteria/*genetics/isolation & purification
MH  - DNA, Bacterial/*genetics
MH  - Drosophila melanogaster/*microbiology
MH  - Feces/microbiology
MH  - Gastrointestinal Tract/microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lactobacillus/genetics/isolation & purification
MH  - Male
MH  - Spatial Analysis
PMC - PMC7894962
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/02/20 06:00
MHDA- 2021/08/24 06:00
PMCR- 2021/02/19
CRDT- 2021/02/19 17:13
PHST- 2020/09/09 00:00 [received]
PHST- 2021/02/05 00:00 [accepted]
PHST- 2021/02/19 17:13 [entrez]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2021/08/24 06:00 [medline]
PHST- 2021/02/19 00:00 [pmc-release]
AID - PONE-D-20-28177 [pii]
AID - 10.1371/journal.pone.0247376 [doi]
PST - epublish
SO  - PLoS One. 2021 Feb 19;16(2):e0247376. doi: 10.1371/journal.pone.0247376. 
      eCollection 2021.