PMID- 33607298
OWN - NLM
STAT- MEDLINE
DCOM- 20220603
LR  - 20220716
IS  - 2210-3244 (Electronic)
IS  - 1672-0229 (Print)
IS  - 1672-0229 (Linking)
VI  - 19
IP  - 5
DP  - 2021 Oct
TI  - Elucidation of the MicroRNA Transcriptome in Western Corn Rootworm Reveals Its 
      Dynamic and Evolutionary Complexity.
PG  - 800-814
LID - S1672-0229(21)00014-0 [pii]
LID - 10.1016/j.gpb.2019.03.008 [doi]
AB  - Diabrotica virgifera virgifera (western corn rootworm, WCR) is one of the most 
      destructive agricultural insect pests in North America. It is highly adaptive to 
      environmental stimuli and crop protection technologies. However, little is known 
      about the underlying genetic basis of WCR behavior and adaptation. More 
      specifically, the involvement of small RNAs (sRNAs), especially microRNAs 
      (miRNAs), a class of endogenous small non-coding RNAs that regulate various 
      biological processes, has not been examined, and the datasets of putative sRNA 
      sequences have not previously been generated for WCR. To achieve a comprehensive 
      collection of sRNA transcriptomes in WCR, we constructed, sequenced, and analyzed 
      sRNA libraries from different life stages of WCR and northern corn rootworm 
      (NCR), and identified 101 conserved precursor miRNAs (pre-miRNAs) in WCR and 
      other Arthropoda. We also identified 277 corn rootworm specific pre-miRNAs. 
      Systematic analyses of sRNA populations in WCR revealed that its sRNA 
      transcriptome, which includes PIWI-interacting RNAs (piRNAs) and miRNAs, 
      undergoes a dynamic change throughout insect development. Phylogenetic analysis 
      of miRNA datasets from model species reveals that a large pool of 
      species-specific miRNAs exists in corn rootworm; these are potentially 
      evolutionarily transient. Comparisons of WCR miRNA clusters to other insect 
      species highlight conserved miRNA-regulated processes that are common to insects. 
      Parallel Analysis of RNA Ends (PARE) also uncovered potential miRNA-guided 
      cleavage sites in WCR. Overall, this study provides a new resource for studying 
      the sRNA transcriptome and miRNA-mediated gene regulation in WCR and other 
      Coleopteran insects.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Yang, Xiaozeng
AU  - Yang X
AD  - Beijing Agro-biotechnology Research Center, Beijing Academy of Agriculture and 
      Forestry Sciences, Beijing 100097, China; Corteva Agriscience, Agriculture 
      Division of DowDuPont, Indianapolis, IN 46268, USA. Electronic address: 
      yangxiaozeng@baafs.net.cn.
FAU - Fishilevich, Elane
AU  - Fishilevich E
AD  - Corteva Agriscience, Agriculture Division of DowDuPont, Indianapolis, IN 46268, 
      USA; University of Nebraska-Lincoln, Department of Entomology, Lincoln, NE 68583, 
      USA.
FAU - German, Marcelo A
AU  - German MA
AD  - Corteva Agriscience, Agriculture Division of DowDuPont, Indianapolis, IN 46268, 
      USA.
FAU - Gandra, Premchand
AU  - Gandra P
AD  - Corteva Agriscience, Agriculture Division of DowDuPont, Indianapolis, IN 46268, 
      USA.
FAU - McEwan, Robert E
AU  - McEwan RE
AD  - Corteva Agriscience, Agriculture Division of DowDuPont, Indianapolis, IN 46268, 
      USA.
FAU - Billion, Andre
AU  - Billion A
AD  - Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of 
      Bioresources, Giessen 35394, Germany.
FAU - Knorr, Eileen
AU  - Knorr E
AD  - Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of 
      Bioresources, Giessen 35394, Germany.
FAU - Vilcinskas, Andreas
AU  - Vilcinskas A
AD  - Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of 
      Bioresources, Giessen 35394, Germany.
FAU - Narva, Kenneth E
AU  - Narva KE
AD  - Corteva Agriscience, Agriculture Division of DowDuPont, Indianapolis, IN 46268, 
      USA. Electronic address: knarva@dow.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210217
PL  - England
TA  - Genomics Proteomics Bioinformatics
JT  - Genomics, proteomics & bioinformatics
JID - 101197608
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - *Coleoptera/genetics
MH  - *MicroRNAs/genetics
MH  - Phylogeny
MH  - Transcriptome
MH  - Zea mays/genetics
PMC - PMC9170749
OTO - NOTNLM
OT  - MicroRNA
OT  - PIWI-interacting RNA
OT  - Parallel Analysis of RNA Ends
OT  - Small RNA
OT  - Western corn rootworm
EDAT- 2021/02/20 06:00
MHDA- 2022/06/07 06:00
PMCR- 2021/02/17
CRDT- 2021/02/19 20:11
PHST- 2018/04/30 00:00 [received]
PHST- 2018/11/21 00:00 [revised]
PHST- 2019/03/28 00:00 [accepted]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2022/06/07 06:00 [medline]
PHST- 2021/02/19 20:11 [entrez]
PHST- 2021/02/17 00:00 [pmc-release]
AID - S1672-0229(21)00014-0 [pii]
AID - 10.1016/j.gpb.2019.03.008 [doi]
PST - ppublish
SO  - Genomics Proteomics Bioinformatics. 2021 Oct;19(5):800-814. doi: 
      10.1016/j.gpb.2019.03.008. Epub 2021 Feb 17.