PMID- 33607589
OWN - NLM
STAT- MEDLINE
DCOM- 20210427
LR  - 20220531
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 124
DP  - 2021 Apr
TI  - PELP1 promotes the expression of RUNX2 via the ERK pathway during the osteogenic 
      differentiation of human periodontal ligament stem cells.
PG  - 105078
LID - S0003-9969(21)00041-8 [pii]
LID - 10.1016/j.archoralbio.2021.105078 [doi]
AB  - OBJECTIVE: The aim of this study was to determine the physiological function and 
      mechanism of proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) in the 
      osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) at 
      the molecular level in vitro. DESIGN: During the osteogenic differentiation of 
      hPDLSCs, the change of PELP1 and the osteogenic commitment markers runt-related 
      transcription factor 2(RUNX2), alkaline phosphatase (ALP) and osteocalcin (OCN) 
      were monitored by quantitative real-time PCR (qRT-PCR) and western blots. To 
      elucidate how PELP1 regulates RUNX2, the expression of RUNX2, the phosphorylation 
      of extracellular regulated protein kinases (ERK) and subcellular location of 
      PELP1 were detected under conditions that PELP1 was either knockdown by specific 
      siRNA or overexpressed. A pharmacological inhibitor of ERK, U0126 was used while 
      PELP1 was overexpressed, and the expression of RUNX2 was monitored by qRT-PCR. 
      RESULTS: PELP1 was upregulated during the osteogenic differentiation of hPDLSCs. 
      Knockdown of PELP1 suppressed the expression of RUNX2, whereas overexpression of 
      PELP1 increased RUNX2 expression. Moreover, PELP1 knockdown resulted in reduced 
      ERK phosphorylation and RUNX2 expression, and PELP1 overexpression induced RUNX2 
      expression was inhibited by U0126 in the hPDLSCs. CONCLUSIONS: PELP1 regulates 
      the expression of RUNX2 during the osteogenic differentiation of hPDLSCs and that 
      the ERK pathway is involved in this process.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Yan, Yan
AU  - Yan Y
AD  - Department of Endodontics, Beijing Stomatological Hospital, School of 
      Stomatology, Capital Medical University, Beijing, 10050, China. Electronic 
      address: yanandf3enc@163.com.
FAU - Liu, Yu
AU  - Liu Y
AD  - Bejing Zebro Dental Clinic, Beijing, 100097, China.
FAU - Lu, Ran
AU  - Lu R
AD  - Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School 
      of Stomatology, Capital Medical University, Beijing, 100050, China.
LA  - eng
PT  - Journal Article
DEP - 20210211
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (PELP1 protein, human)
RN  - 0 (RUNX2 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Co-Repressor Proteins
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - Humans
MH  - MAP Kinase Signaling System
MH  - *Osteogenesis
MH  - *Periodontal Ligament/metabolism
MH  - Protein Kinases
MH  - Stem Cells/metabolism
MH  - Transcription Factors
OTO - NOTNLM
OT  - Human periodontal ligament stem cells
OT  - Osteogenic differentiation
OT  - Proline-, glutamic acid-, and leucine-rich protein 1
OT  - RUNX2
EDAT- 2021/02/20 06:00
MHDA- 2021/04/28 06:00
CRDT- 2021/02/19 20:19
PHST- 2020/09/29 00:00 [received]
PHST- 2021/01/25 00:00 [revised]
PHST- 2021/02/04 00:00 [accepted]
PHST- 2021/02/20 06:00 [pubmed]
PHST- 2021/04/28 06:00 [medline]
PHST- 2021/02/19 20:19 [entrez]
AID - S0003-9969(21)00041-8 [pii]
AID - 10.1016/j.archoralbio.2021.105078 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2021 Apr;124:105078. doi: 10.1016/j.archoralbio.2021.105078. Epub 
      2021 Feb 11.