PMID- 33608078
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1151
DP  - 2021 Mar 22
TI  - Enzyme-induced Cu(2+)/Cu(+) conversion as the electrochemical signal for 
      sensitive detection of ethyl carbamate.
PG  - 338256
LID - S0003-2670(21)00082-9 [pii]
LID - 10.1016/j.aca.2021.338256 [doi]
AB  - Traditional enzyme-linked immunosorbent assay (t-ELISA) method suffers from its 
      relatively low sensitivity or accuracy in the detection of trace level of analyte 
      in complicated samples. In this work, to extend the application of ELISA in 
      practical samples, a newly electrochemical immunoassay (ECIA) was developed based 
      on an enzyme-induced Cu(2+)/Cu(+) conversion for the determination of ethyl 
      carbamate (EC). Wherein, three rounds of signal transformation-the catalysis of 
      ALP enzyme, the conversion of Cu(2+)/Cu(+) and signal output of square wave 
      voltammetry (SWV), can be realized to obtain higher sensitivity as compared to 
      t-ELISA. The ECIA method combines the advantages of electrochemistry and ELISA, 
      behaving superior detection performance, such as good selectivity, high 
      sensitivity, and low background signal. For the wine samples, the method showed a 
      linear detection range from 2.5 nM to 2.5 x 10(4) nM with a limit of detection of 
      2.28 nM (S/N = 3), which reveals that the ECIA sensor is a promising platform for 
      the detection of trace level of EC in practical samples.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Wu, Long
AU  - Wu L
AD  - National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key 
      Laboratory of Fermentation Engineering (Ministry of Education), College of 
      Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei, 430068, PR 
      China; College of Food Sciences & Engineering, Hainan University, 58 People Road, 
      Haikou, 570228, PR China. Electronic address: longquan.good@163.com.
FAU - Wang, Yasheng
AU  - Wang Y
AD  - National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key 
      Laboratory of Fermentation Engineering (Ministry of Education), College of 
      Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei, 430068, PR 
      China.
FAU - Zhou, Shuhong
AU  - Zhou S
AD  - National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key 
      Laboratory of Fermentation Engineering (Ministry of Education), College of 
      Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei, 430068, PR 
      China.
FAU - Zhu, Yongheng
AU  - Zhu Y
AD  - College of Food Science and Technology, And Laboratory of Quality & Safety Risk 
      Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry 
      of Agriculture, Shanghai Ocean University, Shanghai, 201306, China. Electronic 
      address: yh-zhu@shou.edu.cn.
FAU - Chen, Xiaoqiang
AU  - Chen X
AD  - National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key 
      Laboratory of Fermentation Engineering (Ministry of Education), College of 
      Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei, 430068, PR 
      China. Electronic address: biomed528@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210128
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 3IN71E75Z5 (Urethane)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Copper
MH  - *Electrochemical Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoassay
MH  - Limit of Detection
MH  - *Urethane
OTO - NOTNLM
OT  - Alkaline phosphatase enzyme
OT  - Cu(2+)/Cu(+) conversion
OT  - Electrochemical immunoassay
OT  - Ethyl carbamate
OT  - Wine samples
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/02/21 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/02/20 05:26
PHST- 2020/12/03 00:00 [received]
PHST- 2021/01/18 00:00 [revised]
PHST- 2021/01/24 00:00 [accepted]
PHST- 2021/02/20 05:26 [entrez]
PHST- 2021/02/21 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0003-2670(21)00082-9 [pii]
AID - 10.1016/j.aca.2021.338256 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2021 Mar 22;1151:338256. doi: 10.1016/j.aca.2021.338256. Epub 
      2021 Jan 28.