PMID- 33609542
OWN - NLM
STAT- MEDLINE
DCOM- 20211007
LR  - 20230410
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 284
DP  - 2021 Nov 1
TI  - microRNA-6785-5p-loaded human umbilical cord mesenchymal stem cells-derived 
      exosomes suppress angiogenesis and metastasis in gastric cancer via INHBA.
PG  - 119222
LID - S0024-3205(21)00207-1 [pii]
LID - 10.1016/j.lfs.2021.119222 [doi]
AB  - OBJECTIVE: Exosomes (Exos) are known to transfer microRNAs (miRNAs) to 
      participate in human diseases. We aim to identify the role of human umbilical 
      cord mesenchymal stem cells (HUCMSCs)-derived Exos (HUCMSC-Exos) conveying 
      miR-6785-5p in gastric cancer (GC). METHODS: MiR-6785-5p and inhibin subunit beta 
      A (INHBA) expression in GC tissues and cells were determined. GC cells were 
      transfected with the vectors that altered miR-6785-5p or INHBA expression. 
      HUCMSCs were transfected with altered miR-6785-5p or INHBA vectors, and the 
      HUCMSC-Exos were extracted. Then, HUCMSC-Exos were co-cultured with GC cells. The 
      proliferation, migration, invasion, apoptosis and angiogenesis of GC cells were 
      assessed. The binding relationship between miR-6785-5p and INHBA was verified. 
      RESULTS: MiR-6785-5p was down-regulated and INHBA was up-regulated in GC tissues 
      and cells. Elevation of miR-6785-5p or inhibition of INHBA restricted the 
      malignant development of GC cells. HUCMSC-Exos suppressed malignant episodes of 
      GC cells, which could be further enhanced by up-regulated miR-6785-5p or 
      down-regulated INHBA. Elevated INHBA abolished the impacts of up-regulated 
      miR-6785-5p in HUCMSC-Exos on GC cells. INHBA was confirmed as a target gene of 
      miR-6785-5p. CONCLUSION: HUCMSC-Exos containing elevated miR-6785-5p suppress 
      angiogenesis and metastasis in GC via inhibiting INHBA. This study may further 
      the understanding on molecular mechanisms of GC.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Chen, Zonglin
AU  - Chen Z
AD  - Department of General Surgery, The Second Xiangya Hospital, Central South 
      University, Changsha 410008, Hunan, China.
FAU - Xie, Yong
AU  - Xie Y
AD  - Department of General Surgery, The Second Xiangya Hospital, Central South 
      University, Changsha 410008, Hunan, China.
FAU - Chen, Weidong
AU  - Chen W
AD  - Department of General Surgery, The Second Xiangya Hospital, Central South 
      University, Changsha 410008, Hunan, China.
FAU - Li, Tiegang
AU  - Li T
AD  - Department of General Surgery, The Second Xiangya Hospital, Central South 
      University, Changsha 410008, Hunan, China.
FAU - Chen, Xianyu
AU  - Chen X
AD  - Department of General Surgery, The Second Xiangya Hospital, Central South 
      University, Changsha 410008, Hunan, China. Electronic address: 
      chenxianyu6692@163.com.
FAU - Liu, Bo
AU  - Liu B
AD  - Department of General Surgery, The Second Xiangya Hospital, Central South 
      University, Changsha 410008, Hunan, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20210217
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (MicroRNAs)
RN  - 0 (inhibin beta A subunit)
RN  - 93443-12-0 (Inhibin-beta Subunits)
SB  - IM
RIN - Life Sci. 2023 May 15;321:121603. PMID: 36990906
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Cell Proliferation/genetics
MH  - Down-Regulation/genetics
MH  - Exosomes/*metabolism/ultrastructure
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Inhibin-beta Subunits/genetics/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neovascularization, Pathologic/*genetics
MH  - Stomach Neoplasms/*blood supply/*genetics/pathology
MH  - Umbilical Cord/*cytology
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - Angiogenesis
OT  - Apoptosis
OT  - Cytobiology
OT  - Gastric cancer
OT  - Inhibin subunit beta A
OT  - MicroRNA-6785-5p
EDAT- 2021/02/21 06:00
MHDA- 2021/10/08 06:00
CRDT- 2021/02/20 20:09
PHST- 2020/09/22 00:00 [received]
PHST- 2021/02/04 00:00 [revised]
PHST- 2021/02/09 00:00 [accepted]
PHST- 2021/02/21 06:00 [pubmed]
PHST- 2021/10/08 06:00 [medline]
PHST- 2021/02/20 20:09 [entrez]
AID - S0024-3205(21)00207-1 [pii]
AID - 10.1016/j.lfs.2021.119222 [doi]
PST - ppublish
SO  - Life Sci. 2021 Nov 1;284:119222. doi: 10.1016/j.lfs.2021.119222. Epub 2021 Feb 
      17.