PMID- 33609775
OWN - NLM
STAT- MEDLINE
DCOM- 20211117
LR  - 20211117
IS  - 1878-1519 (Electronic)
IS  - 1569-9048 (Linking)
VI  - 288
DP  - 2021 Jun
TI  - SerpinA3n affects ovalbumin (OVA)-induced asthma in neonatal mice via the 
      regulation of collagen deposition and inflammatory response.
PG  - 103642
LID - S1569-9048(21)00027-6 [pii]
LID - 10.1016/j.resp.2021.103642 [doi]
AB  - OBJECTIVE: To investigate the effects of serine protease inhibitor 3n (SerpinA3n) 
      in a neonatal mouse model of asthma. METHODS: The study utilized a neonatal mouse 
      ovalbumin (OVA) sensitization model of asthma. Wild type (WT) and SerpinA3n(-/-) 
      mice were randomly divided into WT/SerpinA3n(-/-) + saline, WT/SerpinA3n(-/-) + 
      OVA, WT/SerpinA3n(-/-) + OVA + rSerpinA3n (recombinant mouse SerpinA3n protein), 
      and WT/SerpinA3n(-/-) + OVA + DEX (dexamethasone, positive control) groups 
      followed by hematoxylin-eosin (HE) staining, Masson's trichrome stainings, Sircol 
      soluble collagen assay, quantitative real time polymerase chain reaction 
      (qRT-PCR), Western Blot and enzyme linked immunosorbent assay (ELISA). RESULTS: 
      OVA-induced neonatal mice showed the increases in airway hyper-reactivity with 
      the up-regulated total cells, eosinophil, lymphocyte and neutrophil in 
      bronchoalveolar lavage fluid (BALF), which was much higher in WT + OVA + 
      rSerpinA3n group (P < 0.05). SerpinA3n(-/-) suppressed the serum concentrations 
      of total immunoglobulin E (IgE) and OVA-specific IgG1 in OVA-induced asthmatic 
      mice, and alleviated the pathological changes of lung tissues, which was reversed 
      by rSerpinA3n injection (P < 0.05). Besides, WT + OVA group showed more severe in 
      collagen deposition in lung tissues than SerpinA3n(-/-) + OVA group with 
      increased expression of matrix metallopeptidase-2 (MMP-2), MMP-9, Eotaxin-1, 
      Interleukin 5 (IL-5), IL-13 and IL-4 in lung tissues and deceased IL-10 and 
      Interferon-gamma (IFN-gamma) (P < 0.05). Nevertheless, the ameliorating effects of 
      SerpinA3n knockout on OVA-induced asthmatic mice can be reversed by rSerpinA3n. 
      CONCLUSION: SerpinA3n knockout can attenuate airway hyper-reactivity, mitigate 
      inflammatory responses and reduce collagen deposition in lung tissues of neonatal 
      mice with asthma.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Zhang, Hai-Tao
AU  - Zhang HT
AD  - Department of Pediatrics, The People's Hospital of Shouguang, Shouguang, 262700, 
      Shandong, China.
FAU - Wang, Ping
AU  - Wang P
AD  - Department of Pediatrics, The People's Hospital of Shouguang, Shouguang, 262700, 
      Shandong, China.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Pediatrics, The People's Hospital of Shouguang, Shouguang, 262700, 
      Shandong, China.
FAU - Bao, Yong-Bo
AU  - Bao YB
AD  - Department of Pediatrics, Zaozhuang Municipal Hospital, Zaozhuang, 277100, 
      Shandong, China. Electronic address: zhmm77@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210217
PL  - Netherlands
TA  - Respir Physiol Neurobiol
JT  - Respiratory physiology & neurobiology
JID - 101140022
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Serpina3n protein, mouse)
RN  - 0 (Serpins)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Acute-Phase Proteins/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Asthma/blood/chemically induced/immunology/*metabolism
MH  - Collagen/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Inflammation/blood/immunology/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Ovalbumin/*pharmacology
MH  - Serpins/*metabolism
OTO - NOTNLM
OT  - Asthma
OT  - Collagen deposition
OT  - Gene knockout
OT  - Inflammatory response
OT  - SerpinA3n
EDAT- 2021/02/21 06:00
MHDA- 2021/11/18 06:00
CRDT- 2021/02/20 20:10
PHST- 2020/11/30 00:00 [received]
PHST- 2021/02/07 00:00 [revised]
PHST- 2021/02/13 00:00 [accepted]
PHST- 2021/02/21 06:00 [pubmed]
PHST- 2021/11/18 06:00 [medline]
PHST- 2021/02/20 20:10 [entrez]
AID - S1569-9048(21)00027-6 [pii]
AID - 10.1016/j.resp.2021.103642 [doi]
PST - ppublish
SO  - Respir Physiol Neurobiol. 2021 Jun;288:103642. doi: 10.1016/j.resp.2021.103642. 
      Epub 2021 Feb 17.