PMID- 33610067
OWN - NLM
STAT- MEDLINE
DCOM- 20220131
LR  - 20220131
IS  - 1879-0593 (Electronic)
IS  - 1368-8375 (Linking)
VI  - 116
DP  - 2021 May
TI  - Detection of deletions in 1q25, 1p36 and 1pTEL and chromosome 17 aneuploidy in 
      oral epithelial dysplasia and oral squamous cell carcinoma by fluorescence in 
      situ hybridization (FISH).
PG  - 105221
LID - S1368-8375(21)00044-0 [pii]
LID - 10.1016/j.oraloncology.2021.105221 [doi]
AB  - OBJECTIVE: To identify chromosome deletions in 1q25, 1p36 and 1pTEL, and 
      chromosome 17 ploidy status in oral epithelial dysplasia (OED) and oral squamous 
      cell carcinoma (OSCC). MATERIAL AND METHODS: Samples from 57 OED and 63 OSCC were 
      selected. FISH was performed using centromeric probes 17 and n LSIR 1p36/LSI 1q25 
      Dual Color Probe. RESULTS: In OED, deletions were found only in 1pTEL region 
      (29.8%). In OSCC, there was a higher frequency of deletion in 1pTEL (79.4%), 
      followed by 1p36 (73.0%), and 1q25 (20.6%). Advanced TNM clinical stages (III/IV) 
      showed all the deletions studied; at early clinical stages (I/II) of OSCC, 
      deletions were observed only in 1pTEL. The frequency of deletion in 1p36 was 17.0 
      times higher in OSCC at advanced clinical stages (PR: 17.00). The median number 
      of cell nuclei with chromosome 17 aneuploidy was higher in OSCC than in OED 
      (P < 0.001). Early clinical stages of OSCC showed lower median number nuclei with 
      aneuploidy when compared to advanced tumors (P < 0.05). Tumors harboring 
      deletions in 1p36, 1q25 and 1pTEL revealed higher median numbers of 
      trisomic/polysomic nuclei when compared to lesions exhibiting no abnormalities in 
      chromosome 1 (P < 0.05). CONCLUSION: A higher prevalence of chromosomal 
      abnormalities was found in OSCC than in OED, while in OSCC, higher abnormalities 
      were present in lesions with higher TNM staging. 1pTEL deletion and monosomy of 
      chromosome 17 are possible markers for progression of OED to OSCC. 1p36 deletion 
      and trisomy/polysomy of chromosome 17 could be markers of worse prognosis of 
      OSCC.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Barem Rabenhorst, Silvia Helena
AU  - Barem Rabenhorst SH
AD  - Department of Pathology and Forensic Medicine, Molecular Genetics Laboratory 
      (LABGEM), School of Medicine, Federal University of Ceara, Fortaleza, Brazil.
FAU - Lima Verde Osterne, Rafael
AU  - Lima Verde Osterne R
AD  - Department of Pathology, University of Fortaleza / Universidade de Fortaleza, 
      School of Medicine, Fortaleza, Brazil. Electronic address: rlimaverde@unifor.br.
FAU - Weege Nonaka, Cassiano Francisco
AU  - Weege Nonaka CF
AD  - State University of Paraiba (UEPB), Campina Grande, Paraiba, Brazil.
FAU - Montezuma Sales Rodrigues, Andre
AU  - Montezuma Sales Rodrigues A
AD  - University of Fortaleza / Universidade de Fortaleza, School of Dentistry, 
      Fortaleza, Brazil.
FAU - Luiz Maia Nogueira, Renato
AU  - Luiz Maia Nogueira R
AD  - Department of Dental Clinic, Discipline of Oral and Maxillofacial Surgery and 
      Stomatology, Federal University of Ceara School of Dentistry, Fortaleza, Brazil. 
      Oral and Maxillofacial Surgeon, Department of Oral and Maxillofacial Surgery, 
      Memorial Batista Hospital, Fortaleza, Brazil.
FAU - Mario Rodriguez Burbano, Rommel
AU  - Mario Rodriguez Burbano R
AD  - Research Center on Oncology, Institute of Health Sciences, Federal University of 
      Para, Mundurucus Street, 66073-000 Belem, Para, Brazil.
FAU - Barroso Cavalcante, Roberta
AU  - Barroso Cavalcante R
AD  - Department of Oral Pathology, University of Fortaleza / Universidade de 
      Fortaleza, School of Dentistry, Fortaleza, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210217
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
SB  - IM
MH  - Aneuploidy
MH  - *Chromosomes, Human, Pair 17
MH  - Gene Deletion
MH  - Humans
MH  - Hyperplasia
MH  - In Situ Hybridization, Fluorescence
MH  - *Mouth Mucosa/pathology
MH  - *Mouth Neoplasms/genetics
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics
OTO - NOTNLM
OT  - Chromosome 1
OT  - Chromosome 17
OT  - Chromosome deletion
OT  - Mouth neoplasms
OT  - Squamous cell carcinoma
EDAT- 2021/02/21 06:00
MHDA- 2022/02/01 06:00
CRDT- 2021/02/20 20:15
PHST- 2020/06/22 00:00 [retracted]
PHST- 2021/01/07 00:00 [revised]
PHST- 2021/02/02 00:00 [accepted]
PHST- 2021/02/21 06:00 [pubmed]
PHST- 2022/02/01 06:00 [medline]
PHST- 2021/02/20 20:15 [entrez]
AID - S1368-8375(21)00044-0 [pii]
AID - 10.1016/j.oraloncology.2021.105221 [doi]
PST - ppublish
SO  - Oral Oncol. 2021 May;116:105221. doi: 10.1016/j.oraloncology.2021.105221. Epub 
      2021 Feb 17.