PMID- 3361167
OWN - NLM
STAT- MEDLINE
DCOM- 19880602
LR  - 20191022
IS  - 0265-6736 (Print)
IS  - 0265-6736 (Linking)
VI  - 4
IP  - 2
DP  - 1988 Mar-Apr
TI  - The effect of hyperthermia on murine leukaemia cells in combination with 
      photodynamic therapy.
PG  - 203-9
AB  - Mouse leukaemia L1210 cells were subjected to hyperthermia (43.0 +/- 0.05 degrees 
      C, water bath) and photodynamic therapy (50 micrograms/ml haematoporphyrin 
      derivative (HpD), 630 +/- 5 nm at 0.1 mW/cm2) for varying lengths of treatment 
      time in different sequences. Dose-response curves showed that the two modalities 
      interact to make the combination more cytotoxic than the sum of the separate 
      individual treatments, and that a determining factor is the intracellular 
      concentration of HpD of tumour cells during hyperthermia. High cytotoxic 
      (synergistic) effects of photodynamic therapy (PDT) were obtained when PDT was 
      performed after hyperthermia following HpD administration. However, only additive 
      cytotoxic effects were found, when hyperthermia was performed after HpD 
      administration and PDT. These cytotoxic data were supported by DNA damage (the 
      increase in single-stranded DNA) as revealed by flow cytometry in cells stained 
      with acridine orange (AO). These in vitro experiments suggest that clinical 
      applications of PDT after hyperthermia with HpD injection might be useful.
FAU - Miyoshi, N
AU  - Miyoshi N
AD  - Department of Pathology, Fukui Medical School, Japan.
FAU - Matsumoto, N
AU  - Matsumoto N
FAU - Hisazumi, H
AU  - Hisazumi H
FAU - Fukuda, M
AU  - Fukuda M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Hyperthermia
JT  - International journal of hyperthermia : the official journal of European Society 
      for Hyperthermic Oncology, North American Hyperthermia Group
JID - 8508395
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Combined Modality Therapy
MH  - DNA Damage
MH  - *Hematoporphyrin Photoradiation
MH  - Hot Temperature/*therapeutic use
MH  - Leukemia L1210/drug therapy/*therapy
MH  - Mice
MH  - *Photochemotherapy
MH  - Tumor Cells, Cultured/drug effects
EDAT- 1988/03/01 00:00
MHDA- 1988/03/01 00:01
CRDT- 1988/03/01 00:00
PHST- 1988/03/01 00:00 [pubmed]
PHST- 1988/03/01 00:01 [medline]
PHST- 1988/03/01 00:00 [entrez]
AID - 10.3109/02656738809029310 [doi]
PST - ppublish
SO  - Int J Hyperthermia. 1988 Mar-Apr;4(2):203-9. doi: 10.3109/02656738809029310.