PMID- 33613105
OWN - NLM
STAT- MEDLINE
DCOM- 20220114
LR  - 20220114
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 17
IP  - 2
DP  - 2021
TI  - 17beta-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female 
      mice against sepsis.
PG  - 460-474
LID - 10.7150/ijbs.53050 [doi]
AB  - Sepsis is a common serious clinical infectious disease accompanied by more severe 
      injuries and higher mortality rates in men than women. The much higher level of 
      17beta-estradiol (E(2)) in female is one of the significant reasons for better 
      sepsis resistance ability. Trained immunity is a novel way to fight against 
      infection by improving innate immunity. However, whether beta-glucan-induced trained 
      immunity can promote macrophage phagocytosis to clear infections in early sepsis 
      has not been clarified. And whether E(2) involved in this process needs further 
      investigation. Symptoms among male, female and ovariectomized (OVX) C57BL/6 mice 
      in early sepsis were detected. The effect of trained immunity on macrophage 
      LC3B-associated phagocytosis (LAP) and the mechanism of E(2) functioned in this 
      process have also been explored. We demonstrated compared with male mice, female 
      has significantly more mild symptoms and more reactive oxygen species (ROS) 
      production and stronger NADPH oxidase 2 (NOX2) expression in the macrophage of 
      major organs. In contrary, these characteristics are disappeared in OVX mice. 
      Furthermore, in macrophage cell lines and primary bone marrow- derived 
      macrophages (BMDMs), beta-glucan-induced trained immunity can increase ROS 
      production by activating NOX2 to promote macrophage LAP. E(2) can up-regulate 
      RUBICON through estrogen receptor alpha (ERalpha) to further facilitate macrophage LAP. 
      These results indicated that trained immunity can improve sepsis resistance 
      ability by stimulating macrophage LAP. E(2) can boost ROS production and RUBICON 
      expression to further promote macrophage LAP, which can provide a new perspective 
      to recognize the mechanism of trained immunity in gender differences when 
      responding to sepsis.
CI  - (c) The author(s).
FAU - Sun, Zhiheng
AU  - Sun Z
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
FAU - Qu, Junxing
AU  - Qu J
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
FAU - Xia, Xiaoyu
AU  - Xia X
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
FAU - Pan, Yuchen
AU  - Pan Y
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
FAU - Liu, Xinghan
AU  - Liu X
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
FAU - Liang, Huaping
AU  - Liang H
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Department of Wound 
      Infection and Drug, Daping Hospital, Army Medical University, Chongqing, China.
FAU - Dou, Huan
AU  - Dou H
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
AD  - Jiangsu Key Laboratory of Molecular Medicine, Division of Immunology, Medical 
      School, Nanjing University, Nanjing, China.
FAU - Hou, Yayi
AU  - Hou Y
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing, China.
AD  - Jiangsu Key Laboratory of Molecular Medicine, Division of Immunology, Medical 
      School, Nanjing University, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210101
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Estradiol/*pharmacology
MH  - Female
MH  - *Immunity
MH  - Mice
MH  - *Phagocytosis
PMC - PMC7893586
OTO - NOTNLM
OT  - LAP
OT  - ROS
OT  - RUBICON
OT  - estradiol
OT  - sepsis
OT  - trained immunity
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2021/02/23 06:00
MHDA- 2022/01/15 06:00
PMCR- 2021/01/01
CRDT- 2021/02/22 05:52
PHST- 2020/09/09 00:00 [received]
PHST- 2020/12/02 00:00 [accepted]
PHST- 2021/02/22 05:52 [entrez]
PHST- 2021/02/23 06:00 [pubmed]
PHST- 2022/01/15 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - ijbsv17p0460 [pii]
AID - 10.7150/ijbs.53050 [doi]
PST - epublish
SO  - Int J Biol Sci. 2021 Jan 1;17(2):460-474. doi: 10.7150/ijbs.53050. eCollection 
      2021.