PMID- 33614446
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210223
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 14
IP  - 2
DP  - 2021
TI  - Protective effects of human umbilical cord mesenchymal stem cells on retinal 
      ganglion cells in mice with acute ocular hypertension.
PG  - 194-199
LID - 10.18240/ijo.2021.02.03 [doi]
AB  - AIM: To observe the protective effect of human umbilical cord mesenchymal stem 
      cells (hucMSCs) on retinal ganglion cells (RGCs) injury in mice with acute ocular 
      hypertension (AOH). METHODS: Fifty-six adult male C57BL/6 mice were randomly 
      divided into four groups: normal group, AOH group, hucMSCs group, normal saline 
      (NS) group. Left eye of mice was induced by 90 mm Hg intraocular pressure for 1h 
      to establish AOH model. hucMSCs 1x10(5)/microL, 1 microL or NS 1 microL was injected into the 
      vitreous body the next day. CM-Dil fluorescent dye was used to label the 3(rd) 
      generation of hucMSCs, for tracing the cells in the vitreous cavity of mice. 
      Seven days after the model established, hematoxylin-eosin (HE) staining was used 
      to observe the thickness of the inner retina layer in four groups. Numbers and 
      loss rate of RGCs were evaluated by counting Brn-3a positive cells stained by 
      immunofluorescencein. RESULTS: On the 7(th) day after AOH established, labeled 
      hucMSCs were found in the vitreous cavity. HE staining showed that the thickness 
      of retinal inner layer in AOH group was significantly lower than that in normal 
      group and hucMSCs group (P<0.05), same as that in NS group (P>0.05). Compared 
      with AOH group, the RGCs in normal group was significantly higher; RGCs number 
      increased in hucMSCs group and the loss rate was lower (P<0.05). Injection of NS 
      had no protective effect on RGCs. CONCLUSION: In AOH mouse model, vitreous 
      injection of hucMSCs have shown a protection for RGCs.
CI  - International Journal of Ophthalmology Press.
FAU - Liu, Rui
AU  - Liu R
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
AD  - Department of Ophthalmology, Shenzhen Hospital, Southern Medical University, 
      Shenzhen 518100, Guangdong Province, China.
FAU - Shi, Qi
AU  - Shi Q
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
FAU - Yang, Hong
AU  - Yang H
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
FAU - Sha, Xiao-Yuan
AU  - Sha XY
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
FAU - Yu, Guo-Cheng
AU  - Yu GC
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
FAU - Liu, Lian
AU  - Liu L
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
FAU - Zhong, Jing-Xiang
AU  - Zhong JX
AD  - Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 
      Guangzhou 510630, Guangdong Province, China.
LA  - eng
PT  - Journal Article
DEP - 20210218
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC7840375
OTO - NOTNLM
OT  - acute ocular hypertension
OT  - glaucoma
OT  - human umbilical cord mesenchymal stem cells
OT  - retinal ganglion cells
EDAT- 2021/02/23 06:00
MHDA- 2021/02/23 06:01
PMCR- 2021/02/18
CRDT- 2021/02/22 05:57
PHST- 2020/03/27 00:00 [received]
PHST- 2020/09/03 00:00 [accepted]
PHST- 2021/02/22 05:57 [entrez]
PHST- 2021/02/23 06:00 [pubmed]
PHST- 2021/02/23 06:01 [medline]
PHST- 2021/02/18 00:00 [pmc-release]
AID - ijo-14-02-194 [pii]
AID - 10.18240/ijo.2021.02.03 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2021 Feb 18;14(2):194-199. doi: 10.18240/ijo.2021.02.03. 
      eCollection 2021.