PMID- 33616025
OWN - NLM
STAT- MEDLINE
DCOM- 20211129
LR  - 20240226
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 43
IP  - 7
DP  - 2021 Jul
TI  - Reelin promotes oligodendrocyte precursors migration via the Wnt/beta-catenin 
      signaling pathway.
PG  - 543-552
LID - 10.1080/01616412.2021.1888604 [doi]
AB  - Objectives: The extracellular matrix glycoprotein Reelin plays an important role 
      in the development of the central nervous system and is involved in neurogenesis, 
      neuronal polarization and migration. Although it has been reported that Reelin 
      and its receptor are expressed in oligodendrocyte precursors (OPCs), the main 
      functions and possible mechanism of Reelin in OPCs remain unclear.Methods: In 
      this study, immunofluorescence staining was used to detect the expressions of 
      A2B5, PDGFRalpha, Reelin, VLDLR and Dab1 in OPCs. The expression of p-Dab1 in OPCs 
      which was treated with Reelin at different concentrations was assayed by western 
      blot. Effects of Reelin on the proliferation of OPCs was measured by EdU and 
      CCK-8. Annexin V-FITC/PI assayed the effect of Reelin on the apoptosis of OPCs. 
      Effects of Reelin on the migration ability of OPCs were detected by the scratch 
      test and transwell experiments. Immunoblotting was used to measure the activation 
      of Wnt/beta-catenin signaling with Reelin, while transwell experiments were 
      performed to verify the migration of OPCs under the activation of Wnt/beta-catenin 
      signaling.Results: Results showed that the receptor of Reelin, very-low-density 
      lipoprotein receptor (VLDLR), and its adaptor protein, Dab1, are highly expressed 
      in A2B5/PDGFRalpha double-positive OPCs. Recombinant Reelin protein promoted OPCs 
      migration in vitro but had no obvious effects on proliferation or apoptosis. 
      Reelin also promoted the phosphorylation of Dab1 and increased the expression of 
      beta-catenin in OPCs. WIKI4, an inhibitor of Wnt/beta-catenin signaling, suppressed the 
      migration of OPCs induced by Reelin.Conclusion: The present study indicated that 
      Reelin promotes OPCs migration via the Wnt/beta-catenin pathway.
FAU - Liu, Yaping
AU  - Liu Y
AD  - Laboratory of National Experimental Teaching and Demonstration Center of Basic 
      Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
FAU - Wang, Yuanyuan
AU  - Wang Y
AD  - Pediatrics, Nanjing Tongren Hospital, Nanjing, Jiangsu, PRC.
FAU - Yuan, Wen
AU  - Yuan W
AD  - Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of 
      Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
FAU - Dong, Fuxing
AU  - Dong F
AD  - Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of 
      Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
FAU - Zhen, Fei
AU  - Zhen F
AD  - Department of Pathology, Hongze District People's Hospital, Huai 'an, Jiangsu, 
      PRC.
FAU - Liu, Jing
AU  - Liu J
AD  - Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of 
      Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
FAU - Yang, Lihua
AU  - Yang L
AD  - Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of 
      Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
FAU - Qu, Xuebin
AU  - Qu X
AD  - Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of 
      Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
FAU - Yao, Ruiqin
AU  - Yao R
AD  - Department of Cell Biology and Neurobiology, Xuzhou Key Laboratory of 
      Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu, PRC.
LA  - eng
PT  - Journal Article
DEP - 20210221
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Reelin Protein)
RN  - 0 (Reln protein, rat)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Animals
MH  - Cell Adhesion Molecules, Neuronal/*metabolism
MH  - Cell Movement/physiology
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurogenesis/physiology
MH  - Neurons/metabolism
MH  - Oligodendrocyte Precursor Cells/*metabolism
MH  - Phosphorylation
MH  - Rats, Sprague-Dawley
MH  - Reelin Protein
MH  - Serine Endopeptidases/*metabolism
MH  - Wnt Signaling Pathway/*physiology
MH  - Rats
OTO - NOTNLM
OT  - OPCs
OT  - Reelin
OT  - Wnt/beta-catenin
OT  - migration
EDAT- 2021/02/23 06:00
MHDA- 2021/11/30 06:00
CRDT- 2021/02/22 08:51
PHST- 2021/02/23 06:00 [pubmed]
PHST- 2021/11/30 06:00 [medline]
PHST- 2021/02/22 08:51 [entrez]
AID - 10.1080/01616412.2021.1888604 [doi]
PST - ppublish
SO  - Neurol Res. 2021 Jul;43(7):543-552. doi: 10.1080/01616412.2021.1888604. Epub 2021 
      Feb 21.