PMID- 33617497
OWN - NLM
STAT- MEDLINE
DCOM- 20210224
LR  - 20210224
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 83
IP  - 4
DP  - 2021 Feb
TI  - Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for 
      phenolic compounds removal.
PG  - 906-921
LID - 10.2166/wst.2021.024 [doi]
AB  - Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized 
      by Fourier transform infrared spectrometry and X-ray diffraction. Polyphenol 
      oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and 
      covalent binding (CPPO). Taguchi method was applied in the optimization of 
      immobilization conditions resulting in the highest enzyme activity of 16.37 x 
      10(3) and 8.92 x 10(3)U/g for APPO and CPPO, respectively. APPO enzyme activity 
      was higher than that of CPPO, while CPPO showed the higher enzyme loading 
      capacity than that of APPO. The removal percentage of phenolic compound, 
      including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by 
      immobilized PPO was also explored. The results indicated that APPO was more 
      efficient in phenolic compounds removal than CPPO. APPO contributed to a quick 
      removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP 
      could reach 69.3 +/- 4.2%, 89.8 +/- 2.5% and 93.8 +/- 1.7% within 2 h, respectively. 
      The order of removal percentage of phenolic compounds for both immobilized PPO 
      was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage 
      of 2,4-DCP reached 73.2 +/- 2.6% and 60.3 +/- 1.5% for APPO and CPPO, respectively. 
      The results introduced a novel support for PPO immobilization, and the 
      immobilized PPO had great potential in wastewater treatment.
FAU - Zhong, Lian
AU  - Zhong L
AD  - College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 
      266100, China.
FAU - Li, Jin
AU  - Li J
AD  - Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean 
      University of China, Qingdao 266100, China E-mail: lijin@ouc.edu.cn; College of 
      Environmental Science and Engineering, Ocean University of China, Qingdao 266100, 
      China.
FAU - Tian, Dawei
AU  - Tian D
AD  - College of Environmental Science and Engineering, Ocean University of China, 
      Qingdao 266100, China.
FAU - Cai, Jun
AU  - Cai J
AD  - Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key 
      Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 
      430068, China.
FAU - Wang, Han
AU  - Wang H
AD  - College of Environmental Science and Engineering, Ocean University of China, 
      Qingdao 266100, China.
FAU - Ma, Qimin
AU  - Ma Q
AD  - Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean 
      University of China, Qingdao 266100, China E-mail: lijin@ouc.edu.cn; College of 
      Environmental Science and Engineering, Ocean University of China, Qingdao 266100, 
      China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Aluminum Silicates)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Minerals)
RN  - 0 (Phenols)
RN  - 0 (rectorite)
RN  - 9012-76-4 (Chitosan)
RN  - EC 1.10.3.1 (Catechol Oxidase)
SB  - IM
MH  - Aluminum Silicates
MH  - Catechol Oxidase
MH  - *Chitosan
MH  - Enzymes, Immobilized
MH  - Hydrogen-Ion Concentration
MH  - Minerals
MH  - Phenols
EDAT- 2021/02/23 06:00
MHDA- 2021/02/25 06:00
CRDT- 2021/02/22 17:11
PHST- 2021/02/22 17:11 [entrez]
PHST- 2021/02/23 06:00 [pubmed]
PHST- 2021/02/25 06:00 [medline]
AID - 10.2166/wst.2021.024 [doi]
PST - ppublish
SO  - Water Sci Technol. 2021 Feb;83(4):906-921. doi: 10.2166/wst.2021.024.