PMID- 33618313
OWN - NLM
STAT- MEDLINE
DCOM- 20210421
LR  - 20210421
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 775
DP  - 2021 Jun 25
TI  - Uptake and effects of graphene oxide nanomaterials alone and in combination with 
      polycyclic aromatic hydrocarbons in zebrafish.
PG  - 145669
LID - S0048-9697(21)00737-3 [pii]
LID - 10.1016/j.scitotenv.2021.145669 [doi]
AB  - Because of its surface characteristics, once in the aquatic environment, graphene 
      could act as a carrier of pollutants, such as polycyclic aromatic hydrocarbons 
      (PAHs), to aquatic organisms. In this study we aimed to (1) assess the capacity 
      of graphene oxide (GO) to sorb PAHs and (2) to evaluate the toxicity of GO alone 
      and in combination with PAHs on zebrafish embryos and adults. GO showed a high 
      sorption capacity for benzo(a)pyrene (B(a)P) (98% of B(a)P sorbed from a nominal 
      concentration of 100 mug/L) and for other PAHs of the water accommodated fraction 
      (WAF) of a naphthenic North Sea crude oil, depending on their log K(ow) (95.7% of 
      phenanthrene, 84.4% of fluorene and 51.5% of acenaphthene). In embryos exposed to 
      different GO nanomaterials alone and with PAHs, no significant mortality was 
      recorded for any treatment. Nevertheless, malformation rate increased 
      significantly in embryos exposed to the highest concentrations (5 or 10 mg/L) of 
      GO and reduced GO (rGO) alone and with sorbed B(a)P (GO-B(a)P). On the other 
      hand, adults were exposed for 21 days to 2 mg/L of GO, GO-B(a)P and GO co-exposed 
      with WAF (GO + WAF) and to 100 mug/L B(a)P. Fish exposed to GO presented GO in the 
      intestine lumen and liver vacuolisation. Transcription level of genes related to 
      cell cycle regulation and oxidative stress was not altered, but the slight 
      up-regulation of cyp1a measured in fish exposed to B(a)P for 3 days resulted in a 
      significantly increased EROD activity. Fish exposed to GO-B(a)P and to B(a)P for 
      3 days and to GO + WAF for 21 days showed significantly higher catalase activity 
      in the gills than control fish. Significantly lower acetylcholinesterase 
      activity, indicating neurotoxic effects, was also observed in all fish treated 
      for 21 days. Results demonstrated the capacity of GO to carry PAHs and to exert 
      sublethal effects in zebrafish.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Martinez-Alvarez, Ignacio
AU  - Martinez-Alvarez I
AD  - University of Bordeaux, EPOC-LPTC, UMR 5805 CNRS, F-33405 Talence Cedex, France; 
      CBET research group, Dept. of Zoology and Animal Cell Biology, Research Centre 
      for Experimental Marine Biology and Biotechnology PiE and Science and Technology 
      Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, 
      Basque Country, Spain.
FAU - Le Menach, Karyn
AU  - Le Menach K
AD  - University of Bordeaux, EPOC-LPTC, UMR 5805 CNRS, F-33405 Talence Cedex, France.
FAU - Devier, Marie-Helene
AU  - Devier MH
AD  - University of Bordeaux, EPOC-LPTC, UMR 5805 CNRS, F-33405 Talence Cedex, France.
FAU - Barbarin, Iranzu
AU  - Barbarin I
AD  - POLYMAT and University of the Basque Country UPV/EHU, Joxe Mari Korta Center - 
      Avda. Tolosa, 72, 20018 San Sebastian, Spain.
FAU - Tomovska, Radmila
AU  - Tomovska R
AD  - POLYMAT and University of the Basque Country UPV/EHU, Joxe Mari Korta Center - 
      Avda. Tolosa, 72, 20018 San Sebastian, Spain; IKERBASQUE, Basque Foundation of 
      Science, Plaza Euskadi, 5, Bilbao 48009, Spain.
FAU - Cajaraville, Miren P
AU  - Cajaraville MP
AD  - CBET research group, Dept. of Zoology and Animal Cell Biology, Research Centre 
      for Experimental Marine Biology and Biotechnology PiE and Science and Technology 
      Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, 
      Basque Country, Spain.
FAU - Budzinski, Helene
AU  - Budzinski H
AD  - University of Bordeaux, EPOC-LPTC, UMR 5805 CNRS, F-33405 Talence Cedex, France.
FAU - Orbea, Amaia
AU  - Orbea A
AD  - CBET research group, Dept. of Zoology and Animal Cell Biology, Research Centre 
      for Experimental Marine Biology and Biotechnology PiE and Science and Technology 
      Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, 
      Basque Country, Spain. Electronic address: amaia.orbea@ehu.eus.
LA  - eng
PT  - Journal Article
DEP - 20210206
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Animals
MH  - *Graphite/toxicity
MH  - *Nanostructures
MH  - North Sea
MH  - *Polycyclic Aromatic Hydrocarbons/analysis/toxicity
MH  - *Water Pollutants, Chemical/toxicity
MH  - Zebrafish
OTO - NOTNLM
OT  - Adsorption
OT  - Aquatic nanotoxicity
OT  - Carbon based nanomaterials
OT  - Organic pollutants
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/02/23 06:00
MHDA- 2021/04/22 06:00
CRDT- 2021/02/22 20:17
PHST- 2020/10/27 00:00 [received]
PHST- 2021/01/18 00:00 [revised]
PHST- 2021/02/02 00:00 [accepted]
PHST- 2021/02/23 06:00 [pubmed]
PHST- 2021/04/22 06:00 [medline]
PHST- 2021/02/22 20:17 [entrez]
AID - S0048-9697(21)00737-3 [pii]
AID - 10.1016/j.scitotenv.2021.145669 [doi]
PST - ppublish
SO  - Sci Total Environ. 2021 Jun 25;775:145669. doi: 10.1016/j.scitotenv.2021.145669. 
      Epub 2021 Feb 6.