PMID- 33620442
OWN - NLM
STAT- MEDLINE
DCOM- 20211022
LR  - 20220224
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 368
IP  - 4
DP  - 2021 Mar 3
TI  - Similar solutions to a common challenge: regulation of genes encoding Ralstonia 
      solanacearum xanthine dehydrogenase.
LID - 10.1093/femsle/fnab022 [doi]
LID - fnab022
AB  - The stringent response involves accumulation of (p)ppGpp, and it ensures that 
      survival is prioritized. Production of (p)ppGpp requires purine synthesis, and 
      upregulation of an operon that encodes the purine salvage enzyme xanthine 
      dehydrogenase (Xdh) has been observed during stringent response in some bacterial 
      species, where direct binding of ppGpp to a TetR-family transcription factor is 
      responsible for increased xdh gene expression. We show here that the plant 
      pathogen Ralstonia solanacearum has a regulatory system in which the LysR-family 
      transcription factor XanR controls expression of the xan operon; this operon 
      encodes Xdh as well as other enzymes involved in purine salvage, which favor 
      accumulation of xanthine. XanR bound upstream of the xan operon, a binding that 
      was attenuated on addition of either ppGpp or cyclic di-guanosine monophosphate 
      (c-di-GMP). Using a reporter in which enhanced green fluorescent protein (EGFP) 
      is expressed under control of a modified xan promoter, XanR was shown to repress 
      EGFP production. Our data suggest that R. solanacearum features a regulatory 
      mechanism in which expression of genes encoding purine salvage enzymes is 
      controlled by a transcription factor that belongs to a different protein family, 
      yet performs similar regulatory functions.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.
FAU - Sivapragasam, Smitha
AU  - Sivapragasam S
AD  - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 
      70803, USA.
FAU - Ghosh, Arpita
AU  - Ghosh A
AD  - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 
      70803, USA.
FAU - Kumar, Sanjay
AU  - Kumar S
AD  - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 
      70803, USA.
FAU - Johnson, Dante T
AU  - Johnson DT
AD  - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 
      70803, USA.
FAU - Grove, Anne
AU  - Grove A
AD  - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 
      70803, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Ligands)
RN  - 0 (Purines)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 1AVZ07U9S7 (Xanthine)
RN  - 38918-96-6 (Guanosine Pentaphosphate)
RN  - EC 1.17.1.4 (Xanthine Dehydrogenase)
SB  - IM
MH  - Binding Sites/genetics
MH  - *Gene Expression Regulation, Bacterial
MH  - Guanosine Pentaphosphate/metabolism
MH  - Ligands
MH  - Models, Molecular
MH  - Operon/genetics
MH  - Purines/metabolism
MH  - Ralstonia solanacearum/enzymology/genetics/metabolism/*physiology
MH  - Repressor Proteins/chemistry/metabolism
MH  - Transcription Factors/chemistry/metabolism
MH  - Xanthine/metabolism
MH  - Xanthine Dehydrogenase/*genetics/metabolism
PMC - PMC8324234
OTO - NOTNLM
OT  - Ralstonia solanacearum
OT  - (p)ppGpp
OT  - LTTR
OT  - gene regulation
OT  - purine salvage
OT  - xanthine dehydrogenase
EDAT- 2021/02/24 06:00
MHDA- 2022/02/24 06:00
PMCR- 2022/02/23
CRDT- 2021/02/23 12:15
PHST- 2020/07/21 00:00 [received]
PHST- 2021/02/19 00:00 [accepted]
PHST- 2021/02/24 06:00 [pubmed]
PHST- 2022/02/24 06:00 [medline]
PHST- 2021/02/23 12:15 [entrez]
PHST- 2022/02/23 00:00 [pmc-release]
AID - 6147036 [pii]
AID - fnab022 [pii]
AID - 10.1093/femsle/fnab022 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2021 Mar 3;368(4):fnab022. doi: 10.1093/femsle/fnab022.