PMID- 33625598
OWN - NLM
STAT- MEDLINE
DCOM- 20210528
LR  - 20210528
IS  - 1617-4623 (Electronic)
IS  - 1617-4623 (Linking)
VI  - 296
IP  - 3
DP  - 2021 May
TI  - Universal fluorescence in situ hybridization (FISH) protocol for mapping 
      repetitive DNAs in insects and other arthropods.
PG  - 513-526
LID - 10.1007/s00438-021-01765-2 [doi]
AB  - Repetitive DNAs comprise large portion of eukaryote genomes. In genome projects, 
      the assembly of repetitive DNAs is challenging due to the similarity between 
      repeats, which generate ambiguities for alignment. Fluorescence in situ 
      hybridization (FISH) is a powerful technique for the physical mapping of various 
      sequences on chromosomes. This technique is thus very helpful in chromosome-based 
      genome assemblies, providing information on the fine architecture of genomes and 
      their evolution. However, various protocols are currently used for FISH mapping, 
      most of which are relatively laborious and expensive, or work properly only with 
      a specific type of probes or sequences, and there is a need for a universal and 
      affordable FISH protocol. Here we tested a FISH protocol for mapping of different 
      DNA repeats, such as multigene families (rDNAs, U snDNAs, histone genes), 
      satellite DNAs, microsatellites, transposable elements, DOP-PCR products, and 
      telomeric motif (TTAGG)(n), on the chromosomes of various insects and other 
      arthropods. Different cell types and stages obtained from diverse tissues were 
      used. The FISH procedure proved high quality and reliable results in all 
      experiments performed. We obtained data on the chromosomal distribution of DNA 
      repeats in representatives of insects and other arthropods. Thus, our results 
      allow us to conclude that the protocol is universal and requires only time 
      adjustment for chromosome/DNA denaturation. The use of this FISH protocol will 
      facilitate studies focused on understanding the evolution and role of repetitive 
      DNA in arthropod genomes.
FAU - Cabral-de-Mello, Diogo Cavalcanti
AU  - Cabral-de-Mello DC
AUID- ORCID: 0000-0002-4721-2655
AD  - Departamento de Biologia Geral e Aplicada, Instituto de Biociencias, UNESP- 
      Universidade Estadual Paulista, Rio Claro, Sao Paulo, CEP 13506-900, Brazil. 
      cabral.mello@unesp.br.
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske 
      Budejovice, Czech Republic. cabral.mello@unesp.br.
FAU - Marec, Frantisek
AU  - Marec F
AUID- ORCID: 0000-0002-6745-5603
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske 
      Budejovice, Czech Republic.
LA  - eng
GR  - 2017/097319 and 2019/19069-7/Fundacao de Amparo a Pesquisa do Estado de Sao 
      Paulo/
GR  - 17-13713S and 20-13784S/Grantova Agentura Ceske Republiky/
PT  - Journal Article
DEP - 20210224
PL  - Germany
TA  - Mol Genet Genomics
JT  - Molecular genetics and genomics : MGG
JID - 101093320
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Arthropods/*genetics
MH  - Chromosome Mapping/*methods
MH  - DNA/*genetics
MH  - Evolution, Molecular
MH  - Fluorescence
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Insecta/*genetics
MH  - Multigene Family/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Telomere/genetics
OTO - NOTNLM
OT  - Arthropoda
OT  - Chromosomes
OT  - Cytogenetics
OT  - DNA repeats
OT  - FISH protocol
OT  - Genome structure
OT  - Insecta
EDAT- 2021/02/25 06:00
MHDA- 2021/05/29 06:00
CRDT- 2021/02/24 12:13
PHST- 2020/10/25 00:00 [received]
PHST- 2021/01/29 00:00 [accepted]
PHST- 2021/02/25 06:00 [pubmed]
PHST- 2021/05/29 06:00 [medline]
PHST- 2021/02/24 12:13 [entrez]
AID - 10.1007/s00438-021-01765-2 [pii]
AID - 10.1007/s00438-021-01765-2 [doi]
PST - ppublish
SO  - Mol Genet Genomics. 2021 May;296(3):513-526. doi: 10.1007/s00438-021-01765-2. 
      Epub 2021 Feb 24.