PMID- 33625896
OWN - NLM
STAT- MEDLINE
DCOM- 20210824
LR  - 20221220
IS  - 1947-5543 (Electronic)
IS  - 1947-5543 (Linking)
VI  - 19
IP  - 3
DP  - 2021 Jun
TI  - Effect of Thawing Rates and Antioxidants on Semen Cryopreservation in Hu Sheep.
PG  - 204-209
LID - 10.1089/bio.2020.0067 [doi]
AB  - Hu sheep is a valuable sheep breed in China, and semen cryopreservation of Hu 
      sheep is important for sustainable development of the agri-food industry. This 
      study aimed to evaluate the effect of thawing rate and antioxidants (procyanidins 
      [PC] and mitoquinone [MitoQ]) on the quality and antioxidant enzyme activity of 
      post-thaw sperm in Hu sheep. Our results showed that the highest sperm quality 
      was obtained from the group thawed at 70 degrees C for 5 seconds. Furthermore, addition 
      of 150 nM MitoQ in the extender could enhance motility, integrity of the membrane 
      and acrosome, and mitochondrial activity, whereas only sperm motility and 
      membrane integrity were increased with 10 mug/mL of PC supplementation, compared 
      with the control group. Meanwhile, both PC (10 mug/mL) and MitoQ (150 nM) 
      supplementation increased the levels of superoxide dismutase and glutathione 
      peroxidase and decreased the levels of reactive oxygen species and 
      malondialdehyde. In conclusion, the optimal thawing protocol of semen 
      cryopreservation in Hu sheep was 70 degrees C for 5 seconds. MitoQ supplementation 
      (150 mM) in the extender could improve sperm quality and reduce the level of 
      oxidative stress in Hu sheep semen after cryopreservation. Further studies are 
      needed to evaluate fertility of the post-thaw semen using MitoQ.
FAU - Wu, Caifeng
AU  - Wu C
AD  - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of 
      Agriculture Sciences, Shanghai, China.
AD  - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of 
      Agri-genetics and Breeding, Shanghai, China.
FAU - Dai, Jianjun
AU  - Dai J
AD  - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of 
      Agriculture Sciences, Shanghai, China.
AD  - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of 
      Agri-genetics and Breeding, Shanghai, China.
FAU - Zhang, Shushan
AU  - Zhang S
AD  - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of 
      Agriculture Sciences, Shanghai, China.
AD  - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of 
      Agri-genetics and Breeding, Shanghai, China.
FAU - Sun, Lingwei
AU  - Sun L
AD  - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of 
      Agriculture Sciences, Shanghai, China.
AD  - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of 
      Agri-genetics and Breeding, Shanghai, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Animal, Dairy, Veterinary Sciences, Utah State University, Logan, 
      Utah, USA.
FAU - Zhang, Defu
AU  - Zhang D
AD  - Institute of Animal Science and Veterinary Medicine, Shanghai Academy of 
      Agriculture Sciences, Shanghai, China.
AD  - Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of 
      Agri-genetics and Breeding, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20210224
PL  - United States
TA  - Biopreserv Biobank
JT  - Biopreservation and biobanking
JID - 101507284
RN  - 0 (Antioxidants)
RN  - 0 (Cryoprotective Agents)
SB  - IM
EIN - Biopreserv Biobank. 2022 Dec;20(6):579. PMID: 36537735
MH  - Animals
MH  - Antioxidants
MH  - China
MH  - Cryoprotective Agents
MH  - Male
MH  - Semen
MH  - Semen Analysis
MH  - *Semen Preservation
MH  - Sheep
MH  - Sperm Motility
MH  - Spermatozoa
OTO - NOTNLM
OT  - antioxidants
OT  - mitoquinone
OT  - procyanidins
OT  - sperm cryopreservation
OT  - thawing rate
EDAT- 2021/02/25 06:00
MHDA- 2021/08/25 06:00
CRDT- 2021/02/24 17:08
PHST- 2021/02/25 06:00 [pubmed]
PHST- 2021/08/25 06:00 [medline]
PHST- 2021/02/24 17:08 [entrez]
AID - 10.1089/bio.2020.0067 [doi]
PST - ppublish
SO  - Biopreserv Biobank. 2021 Jun;19(3):204-209. doi: 10.1089/bio.2020.0067. Epub 2021 
      Feb 24.