PMID- 33627540
OWN - NLM
STAT- MEDLINE
DCOM- 20210727
LR  - 20211204
IS  - 1347-5223 (Electronic)
IS  - 0009-2363 (Linking)
VI  - 69
IP  - 5
DP  - 2021 May 1
TI  - Arctigenin Triggers Apoptosis and Autophagy via PI3K/Akt/mTOR Inhibition in PC-3M 
      Cells.
PG  - 472-480
LID - 10.1248/cpb.c21-00021 [doi]
AB  - Arctigenin (ARG), a natural lignans compound isolated from Arctium lappa L. In 
      this study, the anti-tumor effect of ARG on prostate cancer cell PC-3M and the 
      mechanism of apoptosis and autophagy induced by phosphatidylinositol 3-kinase 
      (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway were discussed, 
      and further confirmed by the joint treatment of ARG and PI3K inhibitor LY294002. 
      Here, the effect of ARG on cell viability was evaluated in PC-3M cells by Cell 
      Counting Kit-8 reagent (CCK-8) assay. After the treatment of ARG, colony 
      formation assay was used to detect the anti-proliferation effect. Annexin 
      V-fluoresceine isothiocyanate/propidium iodide (FITC/PI) kit and 
      4',6-diamidino-2-phenylindole (DAPI) staining were used to detect the apoptosis 
      level, and cell cycle changes were analyzed by flow cytometry. The expression of 
      autophagy was detected by acridine orange staining. In addition, the expression 
      levels of apoptosis and autophagy-related proteins were analyzed by Western blot. 
      The result showed that different concentrations of ARG inhibited the 
      proliferation of PC-3M cells. DAPI staining and flow cytometry showed that ARG 
      induced PC-3M cell apoptosis and arrested cell in G0/G1 phase. Acridine orange 
      staining showed that ARG induced autophagy in PC-3M cells. Western blot 
      experiments showed that ARG inhibited the expression of Bcl-2, promoted the 
      expression of Bax and cleaved caspase-3. At the same time, the expression of 
      autophagy-related proteins LC3B-II and Beclin-1 increased after ARG treatment, 
      but P62 decreased. In addition, further studies have shown that treatment with 
      LY294002 enhanced the effects of ARG on the expression of proteins associated 
      with apoptosis and autophagy, indicating that ARG may induce apoptosis and 
      autophagy through PI3K/Akt/mTOR pathway.
FAU - Sun, Bai-Ling
AU  - Sun BL
AD  - College of Chinese Medicinal Material, Jilin Agricultural University.
FAU - Cai, En-Bo
AU  - Cai EB
AD  - College of Chinese Medicinal Material, Jilin Agricultural University.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - College of Chinese Medicinal Material, Jilin Agricultural University.
FAU - Wang, Yu
AU  - Wang Y
AD  - College of Chinese Medicinal Material, Jilin Agricultural University.
FAU - Yang, Li-Min
AU  - Yang LM
AD  - College of Chinese Medicinal Material, Jilin Agricultural University.
FAU - Wang, Jing-Yao
AU  - Wang JY
AD  - College of Chinese Medicinal Material, Jilin Agricultural University.
LA  - eng
PT  - Journal Article
DEP - 20210225
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Furans)
RN  - 0 (Lignans)
RN  - 0 (Protein Kinase Inhibitors)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - U76MR9VS6M (arctigenin)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/chemistry/isolation & 
      purification/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Arctium/chemistry
MH  - Autophagy/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drug Screening Assays, Antitumor
MH  - Furans/chemistry/isolation & purification/*pharmacology
MH  - Humans
MH  - Lignans/chemistry/isolation & purification/*pharmacology
MH  - Molecular Conformation
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Protein Kinase Inhibitors/chemistry/isolation & purification/*pharmacology
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/metabolism
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/metabolism
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - PC-3M
OT  - apoptosis
OT  - arctigenin
OT  - autophagy
EDAT- 2021/02/26 06:00
MHDA- 2021/07/28 06:00
CRDT- 2021/02/25 05:35
PHST- 2021/02/26 06:00 [pubmed]
PHST- 2021/07/28 06:00 [medline]
PHST- 2021/02/25 05:35 [entrez]
AID - 10.1248/cpb.c21-00021 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 2021 May 1;69(5):472-480. doi: 10.1248/cpb.c21-00021. 
      Epub 2021 Feb 25.