PMID- 33629307
OWN - NLM
STAT- MEDLINE
DCOM- 20210914
LR  - 20210914
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 25
IP  - 3
DP  - 2021 Feb
TI  - Long non-coding RNA UCA1 mediates proliferation and metastasis of laryngeal 
      squamous cell carcinoma cells via regulating miR-185-5p/HOXA13 axis.
PG  - 1366-1378
LID - 24845 [pii]
LID - 10.26355/eurrev_202102_24845 [doi]
AB  - OBJECTIVE: LncRNA urothelial cancer associated 1 (UCA1) is involved in the 
      development of laryngeal squamous cell carcinoma (LSCC), however, its specific 
      mechanism is not fully clear. PATIENTS AND METHODS: Quantitative reverse 
      transcription-PCR (RT-qPCR) was conducted to determine the expressions of 
      lncRNA-UCA1, miR-185-5p and homeobox A13 (HOXA13) in LSCC tissues and cell lines. 
      Cell Counting Kit-8 assay, colony formation assay, wound healing assay, Transwell 
      and flow cytometry, DIANA-LncBase V2, as well as Starbase, Targetscan, and 
      Dual-Luciferase reporter gene system were conducted to detect and confirm the 
      crosstalk networks among lncRNA-UCA1, miR-185-5p, and HOXA13. RESULTS: The levels 
      of UCA1 and HOXA13 were significantly higher and the expression of miR-185-5p was 
      reduced in LSCC tissues and cell lines. Moreover, miR-185-5p was predicted as a 
      target gene for lncRNA UCA1, while HOXA13 was the target gene for miR-185-5p. 
      UCA1 siRNA inhibited the proliferation and invasion of LSCC cells, moreover, the 
      proliferation and invasion of LSCC cells were suppressed by miR-185-5p mimics but 
      were enhanced by miR-185-5p inhibitor. UCA1 siRNA and overexpressed HOXA13 
      reversed the promotive effects of miR-185-5p inhibitor and inhibitory effects of 
      miR-185-5p mimics on cell proliferation and metastasis, respectively. 
      CONCLUSIONS: The current findings reveal the important role of lncRNA 
      UCA1/miR-185-5p/HOXA13 regulatory network in LSCC cells, and potentially provide 
      new insights into the pathogenesis of LSCC.
FAU - Sun, Y-D
AU  - Sun YD
AD  - Department of Otolaryngology, Hospital of Traditional Chinese Medicine Affiliated 
      to Southwest Medical University, Luzhou, Sichuan Province, China. 
      xingwei_zhouxw@163.com.
FAU - Liu, Q
AU  - Liu Q
FAU - Yang, H-X
AU  - Yang HX
FAU - Tian, L
AU  - Tian L
FAU - Wang, J
AU  - Wang J
FAU - Zeng, L
AU  - Zeng L
FAU - Zhou, X-W
AU  - Zhou XW
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MIRN185 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (homeobox protein HOXA13)
SB  - IM
MH  - Cell Proliferation
MH  - Head and Neck Neoplasms/*metabolism/pathology
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/*metabolism/pathology
EDAT- 2021/02/26 06:00
MHDA- 2021/09/15 06:00
CRDT- 2021/02/25 05:50
PHST- 2021/02/25 05:50 [entrez]
PHST- 2021/02/26 06:00 [pubmed]
PHST- 2021/09/15 06:00 [medline]
AID - 24845 [pii]
AID - 10.26355/eurrev_202102_24845 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2021 Feb;25(3):1366-1378. doi: 
      10.26355/eurrev_202102_24845.