PMID- 33629805
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20231111
IS  - 1751-7915 (Electronic)
IS  - 1751-7915 (Linking)
VI  - 14
IP  - 3
DP  - 2021 May
TI  - Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced 
      CaCO(3) precipitation in bio-based cementitious composites.
PG  - 1044-1059
LID - 10.1111/1751-7915.13752 [doi]
AB  - Microbially induced calcite precipitation (MICP), secreted through biological 
      metabolic activity, secured an imperative position in remedial measures within 
      the construction industry subsequent to ecological, environmental and economical 
      returns. However, this contemporary recurrent healing system is susceptible to 
      microbial depletion in the highly alkaline cementitious environment. Therefore, 
      researchers are probing for alkali resistant calcifying microbes. In the present 
      study, alkaliphilic microbes were isolated from different soil sources and 
      screened for probable CaCO(3) precipitation. Non-ureolytic pathway (oxidation of 
      organic carbon) was adopted for calcite precipitation to eliminate the production 
      of toxic ammonia. For this purpose, calcium lactate Ca(C(3) H(5) O(3) )(2) and 
      calcium acetate Ca(CH(3) COO)(2) were used as CaCO(3) precipitation precursors. 
      The quantification protocol for precipitated CaCO(3) was established to select 
      potent microbial species for implementation in the alkaline cementitious systems 
      as more than 50% of isolates were able to precipitate CaCO(3) . Results suggested 
      80% of potent calcifying strains isolated in this study, portrayed higher calcite 
      precipitation at pH 10 when compared to pH 7. Ten superlative morphologically 
      distinct isolates capable of CaCO(3) production were identified by 16SrRNA 
      sequencing. Sequenced microbes were identified as species of Bacillus, 
      Arthrobacter, Planococcus, Chryseomicrobium and Corynebacterium. Further, 
      microstructure of precipitated CaCO(3) was inspected through scanning electron 
      microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric (TG) analysis. 
      Then, the selected microbes were investigated in the cementitious mortar to rule 
      out any detrimental effects on mechanical properties. These strains showed 
      maximum of 36% increase in compressive strength and 96% increase in flexural 
      strength. Bacillus, Arthrobacter, Corynebacterium and Planococcus genera have 
      been reported as CaCO(3) producers but isolated strains have not yet been 
      investigated in conjunction with cementitious mortar. Moreover, species of 
      Chryseomicrobium and Glutamicibacter were reported first time as calcifying 
      strains.
CI  - (c) 2021 The Authors. Microbial Biotechnology published by Society for Applied 
      Microbiology and John Wiley & Sons Ltd.
FAU - Shaheen, Nafeesa
AU  - Shaheen N
AD  - NUST Institute of Civil Engineering (NICE), School of Civil and Environmental 
      Engineering (SCEE), National University of Sciences and Technology (NUST), Sector 
      H-12, Islamabad, 44000, Pakistan.
FAU - Jalil, Amna
AU  - Jalil A
AD  - Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of 
      Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.
FAU - Adnan, Fazal
AU  - Adnan F
AUID- ORCID: 0000-0002-6624-9285
AD  - Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of 
      Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.
FAU - Arsalan Khushnood, Rao
AU  - Arsalan Khushnood R
AUID- ORCID: 0000-0002-9532-4096
AD  - NUST Institute of Civil Engineering (NICE), School of Civil and Environmental 
      Engineering (SCEE), National University of Sciences and Technology (NUST), Sector 
      H-12, Islamabad, 44000, Pakistan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210225
PL  - United States
TA  - Microb Biotechnol
JT  - Microbial biotechnology
JID - 101316335
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - *Bacillus
MH  - Bacteria/genetics
MH  - *Calcium Carbonate
MH  - Chemical Precipitation
MH  - Feasibility Studies
PMC - PMC8085925
COIS- The authors declare that they have no competing interest.
EDAT- 2021/02/26 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/02/25
CRDT- 2021/02/25 08:40
PHST- 2020/09/20 00:00 [received]
PHST- 2021/01/02 00:00 [accepted]
PHST- 2021/02/26 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/02/25 08:40 [entrez]
PHST- 2021/02/25 00:00 [pmc-release]
AID - MBT213752 [pii]
AID - 10.1111/1751-7915.13752 [doi]
PST - ppublish
SO  - Microb Biotechnol. 2021 May;14(3):1044-1059. doi: 10.1111/1751-7915.13752. Epub 
      2021 Feb 25.