PMID- 33631558
OWN - NLM
STAT- MEDLINE
DCOM- 20210421
LR  - 20220531
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 775
DP  - 2021 Jun 25
TI  - Functional and transcriptomic investigation of laccase activity in the presence 
      of PCB29 identifies two novel enzymes and the multicopper oxidase repertoire of a 
      marine-derived fungus.
PG  - 145818
LID - S0048-9697(21)00885-8 [pii]
LID - 10.1016/j.scitotenv.2021.145818 [doi]
AB  - Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs), that 
      can be detected in a variety of environments including the human body, adversely 
      affecting global health. Bioremediation is an emerging field for the 
      detoxification and removal of environmental pollutants, with novel biocatalysts 
      appropriate for this task being in high demand. In this study, a biobank of novel 
      fungal strains isolated as symbionts of marine invertebrates was screened for 
      their ability to remove 2,4,5-trichlorobiphenyl (PCB29). The most efficient 
      strains were studied further for their ability to express laccase activity, the 
      most commonly associated extracellular activity involved in the removal of 
      aromatic pollutants and encoded in fungi by the enzymatic class of multicopper 
      oxidases (MCOs). The strain expressing the highest laccase activity, Cladosporium 
      sp. TM138-S3, was cultivated in the presence of copper ions in a 12 L bioreactor 
      and two enzymes exhibiting laccase activity were isolated from the culture broth 
      through ion-exchange chromatography. The two enzymes, Lac1 and Lac2, were 
      biochemically characterized and showed similar characteristics, although an 
      improved ability to remove PCB29 (up to 71.2%) was observed for Lac2 in the 
      presence of mediators. In parallel, we performed RNAseq of the strain growing in 
      presence and absence of PCB29 and reconstructed its transcriptome assembly. 
      Functional annotation allowed identifying the MCO repertoire of the fungus, 
      consisting of 13 enzymes. Phylogenetic analysis of Ascomycete MCOs further 
      allowed classifying these enzymes, revealing the diversity of laccase activities 
      in Cladosporium sp. TM138-S3.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Nikolaivits, Efstratios
AU  - Nikolaivits E
AD  - Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School 
      of Chemical Engineering, National Technical University of Athens, Athens, Greece.
FAU - Siaperas, Romanos
AU  - Siaperas R
AD  - Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School 
      of Chemical Engineering, National Technical University of Athens, Athens, Greece.
FAU - Agrafiotis, Andreas
AU  - Agrafiotis A
AD  - Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School 
      of Chemical Engineering, National Technical University of Athens, Athens, Greece.
FAU - Ouazzani, Jamal
AU  - Ouazzani J
AD  - Institut de Chimie des Substances Naturelles, ICSN, CNRS, Gif sur Yvette, France.
FAU - Magoulas, Antonios
AU  - Magoulas A
AD  - Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for 
      Marine Research, Heraklion, Greece.
FAU - Gioti, Alphanastasia
AU  - Gioti Alpha
AD  - Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for 
      Marine Research, Heraklion, Greece.
FAU - Topakas, Evangelos
AU  - Topakas E
AD  - Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School 
      of Chemical Engineering, National Technical University of Athens, Athens, Greece. 
      Electronic address: vtopakas@chemeng.ntua.gr.
LA  - eng
PT  - Journal Article
DEP - 20210212
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - *Ascomycota/metabolism
MH  - Biodegradation, Environmental
MH  - *Laccase/genetics/metabolism
MH  - Phylogeny
MH  - Transcriptome
OTO - NOTNLM
OT  - Bioremediation
OT  - Cladosporium
OT  - Marine-derived fungi
OT  - Multicopper oxidases
OT  - PCBs
OT  - Transcriptome
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/02/26 06:00
MHDA- 2021/04/22 06:00
CRDT- 2021/02/25 20:15
PHST- 2020/12/13 00:00 [received]
PHST- 2021/02/04 00:00 [revised]
PHST- 2021/02/08 00:00 [accepted]
PHST- 2021/02/26 06:00 [pubmed]
PHST- 2021/04/22 06:00 [medline]
PHST- 2021/02/25 20:15 [entrez]
AID - S0048-9697(21)00885-8 [pii]
AID - 10.1016/j.scitotenv.2021.145818 [doi]
PST - ppublish
SO  - Sci Total Environ. 2021 Jun 25;775:145818. doi: 10.1016/j.scitotenv.2021.145818. 
      Epub 2021 Feb 12.