PMID- 33633583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210227
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 12
DP  - 2021
TI  - Serum Metabolomic Profiling to Reveal Potential Biomarkers for the Diagnosis of 
      Fatty Liver Hemorrhagic Syndrome in Laying Hens.
PG  - 590638
LID - 10.3389/fphys.2021.590638 [doi]
LID - 590638
AB  - Fatty liver hemorrhage syndrome (FLHS), a nutritional and metabolic disease that 
      frequently occurs in laying hens, causes serious losses to the poultry industry. 
      Nowadays, the traditional clinical diagnosis of FLHS still has its limitations. 
      Therefore, searching for some metabolic biomarkers and elucidating the metabolic 
      pathway in vivo are useful for the diagnosis and prevention of FLHS. In the 
      present study, a model of FLHS in laying hens induced by feeding a high-energy, 
      low-protein diet was established. Gas chromatography time-of-flight mass 
      spectrometry (GC-TOF-MS) was used to analyze the metabolites in serum at days 40 
      and 80. The result showed that, in total, 40 differential metabolites closely 
      related to the occurrence and development of FLHS were screened and identified, 
      which were mainly associated with lipid metabolism, amino acid metabolism, and 
      energy metabolism pathway disorders. Further investigation of differential 
      metabolites showed 10 potential biomarkers such as 3-hydroxybutyric acid, oleic 
      acid, palmitoleic acid, and glutamate were possessed of high diagnostic values by 
      analyzing receiver operating characteristic (ROC) curves. In conclusion, this 
      study showed that the metabolomic method based on GC-TOF-MS can be used in the 
      clinical diagnosis of FLHS in laying hens and provide potential biomarkers for 
      early risk evaluation of FLHS and further insights into FLHS development.
CI  - Copyright (c) 2021 Guo, Kuang, Zhuang, Jiang, Shi, Huang, Zhou, Xu, Liu, Wu, Hu and 
      Guo.
FAU - Guo, Lianying
AU  - Guo L
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Kuang, Jun
AU  - Kuang J
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Zhuang, Yu
AU  - Zhuang Y
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Jiang, Jialin
AU  - Jiang J
AD  - Jiangxi Biological Vocational College, Nanchang University, Nanchang, China.
FAU - Shi, Yan
AU  - Shi Y
AD  - School of Computer and Information Engineering, Jiangxi Agricultural University, 
      Nanchang, China.
FAU - Huang, Cheng
AU  - Huang C
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Zhou, Changming
AU  - Zhou C
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Xu, Puzhi
AU  - Xu P
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Liu, Ping
AU  - Liu P
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Wu, Cong
AU  - Wu C
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Hu, Guoliang
AU  - Hu G
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
FAU - Guo, Xiaoquan
AU  - Guo X
AD  - Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science 
      and Technology, Jiangxi Agricultural University, Nanchang, China.
LA  - eng
PT  - Journal Article
DEP - 20210209
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC7900428
OTO - NOTNLM
OT  - biomarkers
OT  - diagnosis
OT  - fatty liver hemorrhage syndrome
OT  - gas chromatography time-of-flight mass spectrometry
OT  - metabolomics
OT  - serum
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/02/27 06:00
MHDA- 2021/02/27 06:01
PMCR- 2021/02/09
CRDT- 2021/02/26 06:03
PHST- 2020/08/02 00:00 [received]
PHST- 2021/01/04 00:00 [accepted]
PHST- 2021/02/26 06:03 [entrez]
PHST- 2021/02/27 06:00 [pubmed]
PHST- 2021/02/27 06:01 [medline]
PHST- 2021/02/09 00:00 [pmc-release]
AID - 10.3389/fphys.2021.590638 [doi]
PST - epublish
SO  - Front Physiol. 2021 Feb 9;12:590638. doi: 10.3389/fphys.2021.590638. eCollection 
      2021.