PMID- 33637538
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20220531
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 8
IP  - 2
DP  - 2021 Mar-Apr
TI  - A Role for STOML3 in Olfactory Sensory Transduction.
LID - ENEURO.0565-20.2021 [pii]
LID - 10.1523/ENEURO.0565-20.2021 [doi]
AB  - Stomatin-like protein-3 (STOML3) is an integral membrane protein expressed in the 
      cilia of olfactory sensory neurons (OSNs), but its functional role in this cell 
      type has never been addressed. STOML3 is also expressed in dorsal root ganglia 
      neurons, where it has been shown to be required for normal touch sensation. Here, 
      we extended previous results indicating that STOML3 is mainly expressed in the 
      knob and proximal cilia of OSNs. We additionally showed that mice lacking STOML3 
      have a morphologically normal olfactory epithelium. Because of its presence in 
      the cilia, together with known olfactory transduction components, we hypothesized 
      that STOML3 could be involved in modulating odorant responses in OSNs. To 
      investigate the functional role of STOML3, we performed loose patch recordings 
      from wild-type (WT) and Stoml3 knock-out (KO) OSNs. We found that spontaneous 
      mean firing activity was lower with additional shift in interspike intervals 
      (ISIs) distributions in Stoml3 KOs compared with WT neurons. Moreover, the firing 
      activity in response to stimuli was reduced both in spike number and duration in 
      neurons lacking STOML3 compared with WT neurons. Control experiments suggested 
      that the primary deficit in neurons lacking STOML3 was at the level of 
      transduction and not at the level of action potential generation. We conclude 
      that STOML3 has a physiological role in olfaction, being required for normal 
      sensory encoding by OSNs.
CI  - Copyright (c) 2021 Agostinelli et al.
FAU - Agostinelli, Emilio
AU  - Agostinelli E
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy.
FAU - Gonzalez-Velandia, Kevin Y
AU  - Gonzalez-Velandia KY
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy.
FAU - Hernandez-Clavijo, Andres
AU  - Hernandez-Clavijo A
AUID- ORCID: 0000-0002-4122-3521
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy.
FAU - Kumar Maurya, Devendra
AU  - Kumar Maurya D
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy.
FAU - Xerxa, Elena
AU  - Xerxa E
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy.
FAU - Lewin, Gary R
AU  - Lewin GR
AUID- ORCID: 0000-0002-2890-6352
AD  - Molecular Physiology of Somatic Sensation, Department of Neuroscience, Max 
      Delbruck Center for Molecular Medicine, Berlin D-13122, Germany.
FAU - Dibattista, Michele
AU  - Dibattista M
AD  - Department of Basic Medical Sciences, Neuroscience and Sensory Organs, University 
      of Bari A. Moro, Bari 70124, Italy spifferi@sissa.it menini@sissa.it 
      michele.dibattista@uniba.it.
FAU - Menini, Anna
AU  - Menini A
AUID- ORCID: 0000-0001-9304-0412
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy spifferi@sissa.it menini@sissa.it 
      michele.dibattista@uniba.it.
FAU - Pifferi, Simone
AU  - Pifferi S
AUID- ORCID: 0000-0002-3687-3984
AD  - Neurobiology Group, Scuola Internazionale Superiore di Studi Avanzati (SISSA), 
      Trieste 34136, Italy spifferi@sissa.it menini@sissa.it 
      michele.dibattista@uniba.it.
AD  - Department of Experimental and Clinical Medicine, Universita Politecnica delle 
      Marche, Ancona 60126, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210312
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Stoml3 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cilia
MH  - Membrane Proteins/*genetics
MH  - Mice
MH  - Nerve Tissue Proteins/*genetics
MH  - Olfactory Mucosa
MH  - *Olfactory Receptor Neurons
MH  - *Smell
PMC - PMC7986538
OTO - NOTNLM
OT  - ion channel
OT  - olfactory
OT  - transduction
EDAT- 2021/02/28 06:00
MHDA- 2021/06/22 06:00
PMCR- 2021/03/11
CRDT- 2021/02/27 05:32
PHST- 2020/12/28 00:00 [received]
PHST- 2021/01/25 00:00 [revised]
PHST- 2021/02/08 00:00 [accepted]
PHST- 2021/02/28 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/02/27 05:32 [entrez]
PHST- 2021/03/11 00:00 [pmc-release]
AID - ENEURO.0565-20.2021 [pii]
AID - eN-NWR-0565-20 [pii]
AID - 10.1523/ENEURO.0565-20.2021 [doi]
PST - epublish
SO  - eNeuro. 2021 Mar 12;8(2):ENEURO.0565-20.2021. doi: 10.1523/ENEURO.0565-20.2021. 
      Print 2021 Mar-Apr.