PMID- 33638611
OWN - NLM
STAT- MEDLINE
DCOM- 20220223
LR  - 20220223
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 109
IP  - 9
DP  - 2021 Sep
TI  - 10-hydroxy-2-decenoic acid a bio-immunomodulator in tissue engineering; generates 
      tolerogenic dendritic cells by blocking the toll-like receptor4.
PG  - 1575-1587
LID - 10.1002/jbm.a.37152 [doi]
AB  - Dendritic cells (DCs), in response to the biomaterials, utilize toll-like 
      receptors (TLRs) to become mature or tolerogenic through TLRs-dependent signaling 
      pathways, especially TLR4. Regarding the physicochemical properties of 
      biomaterials, some of such signaling pathways are activated. Unsaturated fatty 
      acids have been explored as an antagonist for TLRs and lead to the tolerogenic 
      phenotype of DCs. Here we showed that, although cultured DCs on both chitosan and 
      Alginate-polyethyleneimine (Alg-PEI) films became fully mature, 
      10-hydroxy-2-decanoic acid (10-HDA), an unsaturated fatty acid found in royal 
      jelly, led to the tolerogenic immunophenotype of DCs on both films. The cultured 
      cells on the films possessed iDCs-like morphology in the presence of 10-HDA. 
      Moreover, 10-HDA expressed lower levels of CD80, CD83, CD86, and HLA-DR, a higher 
      level of IL-10, and lower level of IL-12 in the cultured DCs on both films. 
      Furthermore, HEK293T cells expressing only TLR4 (HEK-TLR4 cells) were co-cultured 
      with LPS, a specific agonist for TLR4, and 10-HDA. The 10-HDA significantly 
      reduced the expression of tumor necrosis factor-a (TNF-alpha) in the HEK-TLR4 cells 
      compared to treated only with LPS. These findings indicate that the 10-HDA acts 
      as an antagonist of TLR4; therefore, potentially can be used in autoimmune 
      diseases and preventing the rejection of biomaterials implantation and allograft 
      transplantation.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Eslami-Kaliji, Farshid
AU  - Eslami-Kaliji F
AD  - Department of Biomedical Engineering, Faculty of Engineering, University of 
      Isfahan, Isfahan, Iran.
AD  - Department of Animal Biotechnology, Cell Science Research Center, Royan Institute 
      for Biotechnology, ACECR, Isfahan, Iran.
FAU - Sarafbidabad, Mohsen
AU  - Sarafbidabad M
AD  - Department of Biomedical Engineering, Faculty of Engineering, University of 
      Isfahan, Isfahan, Iran.
FAU - Kiani-Esfahani, Abbas
AU  - Kiani-Esfahani A
AD  - Department of Animal Biotechnology, Cell Science Research Center, Royan Institute 
      for Biotechnology, ACECR, Isfahan, Iran.
FAU - Mirahmadi-Zare, Seyede Zohreh
AU  - Mirahmadi-Zare SZ
AUID- ORCID: 0000-0003-1898-7969
AD  - Department of Animal Biotechnology, Cell Science Research Center, Royan Institute 
      for Biotechnology, ACECR, Isfahan, Iran.
FAU - Dormiani, Kianoush
AU  - Dormiani K
AD  - Department of Animal Biotechnology, Cell Science Research Center, Royan Institute 
      for Biotechnology, ACECR, Isfahan, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210227
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Immunologic Factors)
RN  - 0 (Toll-Like Receptor 4)
RN  - 765-01-5 (10-hydroxy-2-decenoic acid)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Cytokines/metabolism
MH  - Dendritic Cells/drug effects/*immunology
MH  - Fatty Acids, Monounsaturated/*pharmacology
MH  - HEK293 Cells
MH  - Humans
MH  - *Immune Tolerance/drug effects
MH  - Immunologic Factors/*pharmacology
MH  - Signal Transduction
MH  - Spectroscopy, Fourier Transform Infrared
MH  - *Tissue Engineering
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - 10-HDA
OT  - Immunomodulator
OT  - dendritic cells
OT  - toll-like receptor 4
OT  - unsaturated fatty acid
EDAT- 2021/02/28 06:00
MHDA- 2022/02/24 06:00
CRDT- 2021/02/27 08:36
PHST- 2021/02/03 00:00 [revised]
PHST- 2020/08/18 00:00 [received]
PHST- 2021/02/10 00:00 [accepted]
PHST- 2021/02/28 06:00 [pubmed]
PHST- 2022/02/24 06:00 [medline]
PHST- 2021/02/27 08:36 [entrez]
AID - 10.1002/jbm.a.37152 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2021 Sep;109(9):1575-1587. doi: 10.1002/jbm.a.37152. Epub 
      2021 Feb 27.