PMID- 33638725
OWN - NLM
STAT- MEDLINE
DCOM- 20211206
LR  - 20211214
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Print)
IS  - 1615-7591 (Linking)
VI  - 44
IP  - 6
DP  - 2021 Jun
TI  - Design of experiment (DOE) applied to artificial neural network architecture 
      enables rapid bioprocess improvement.
PG  - 1301-1308
LID - 10.1007/s00449-021-02529-3 [doi]
AB  - Modern bioprocess development employs statistically optimized design of 
      experiments (DOE) and regression modeling to find optimal bioprocess set points. 
      Using modeling software, such as JMP Pro, it is possible to leverage artificial 
      neural networks (ANNs) to improve model accuracy beyond the capabilities of 
      regression models. Herein, we bridge the gap between a DOE skill set and a 
      machine learning skill set by demonstrating a novel use of DOE to systematically 
      create and evaluate ANN architecture using JMP Pro software. Additionally, we run 
      a mammalian cell culture process at historical, one factor at a time, standard 
      least squares regression, and ANN-derived set points. This case study 
      demonstrates the significant differences between one factor at a time bioprocess 
      development, DOE bioprocess development and the relative power of linear 
      regression versus an ANN-DOE hybrid modeling approach.
FAU - Rodriguez-Granrose, Daniel
AU  - Rodriguez-Granrose D
AD  - DiscGenics Inc, Salt Lake City, Utah, USA. daniel@discgenics.com.
AD  - Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL, 
      USA. daniel@discgenics.com.
FAU - Jones, Amanda
AU  - Jones A
AD  - DiscGenics Inc, Salt Lake City, Utah, USA.
FAU - Loftus, Hannah
AU  - Loftus H
AD  - DiscGenics Inc, Salt Lake City, Utah, USA.
FAU - Tandeski, Terry
AU  - Tandeski T
AD  - DiscGenics Inc, Salt Lake City, Utah, USA.
FAU - Heaton, Will
AU  - Heaton W
AD  - DiscGenics Inc, Salt Lake City, Utah, USA.
FAU - Foley, Kevin T
AU  - Foley KT
AD  - DiscGenics Inc, Salt Lake City, Utah, USA.
AD  - Department of Neurosurgery, University of Tennessee Health Science Center, 
      Memphis, TN, USA.
AD  - Semmes-Murphey Clinic, Memphis, TN, USA.
FAU - Silverman, Lara
AU  - Silverman L
AD  - DiscGenics Inc, Salt Lake City, Utah, USA.
AD  - Department of Neurosurgery, University of Tennessee Health Science Center, 
      Memphis, TN, USA.
LA  - eng
GR  - 1451511/National Science Foundation Graduate Research Fellowship/
PT  - Journal Article
DEP - 20210227
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
SB  - IM
MH  - *Models, Biological
MH  - *Neural Networks, Computer
MH  - *Software
PMC - PMC8144078
OTO - NOTNLM
OT  - Artificial neural network
OT  - Bioprocess
OT  - Design of experiments (DOE)
OT  - MachineLearning
OT  - Process modeling
COIS- D Rodriguez-Granrose, H Loftus, A Jones, T Tandeski, W Heaton, K Foley and L 
      Silverman are employees at DiscGenics. D Rodriguez-Granrose, H Loftus, A Jones, T 
      Tandeski, W Heaton, K Foley and L Silverman own stock or stock options in 
      DiscGenics. K Foley is on the board of DiscGenics.
EDAT- 2021/02/28 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/02/27
CRDT- 2021/02/27 12:07
PHST- 2020/09/11 00:00 [received]
PHST- 2021/02/04 00:00 [accepted]
PHST- 2021/02/28 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/02/27 12:07 [entrez]
PHST- 2021/02/27 00:00 [pmc-release]
AID - 10.1007/s00449-021-02529-3 [pii]
AID - 2529 [pii]
AID - 10.1007/s00449-021-02529-3 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2021 Jun;44(6):1301-1308. doi: 
      10.1007/s00449-021-02529-3. Epub 2021 Feb 27.