PMID- 33641784
OWN - NLM
STAT- MEDLINE
DCOM- 20211019
LR  - 20211019
IS  - 1875-5364 (Electronic)
IS  - 1875-5364 (Linking)
VI  - 19
IP  - 2
DP  - 2021 Feb
TI  - Artemisia kruhsiana leaf extract induces autophagic cell death in human prostate 
      cancer cells.
PG  - 134-142
LID - S1875-5364(21)60014-6 [pii]
LID - 10.1016/S1875-5364(21)60014-6 [doi]
AB  - Some species of Artemisia have been reported to induce apoptosis and autophagy, 
      but little is known of the apoptotic and autophagic effects of the stems and 
      leaves of Artemisia kruhsiana Bess. (AkB). This study was conducted to 
      investigate the antioxidant and anti-autophagic effects of the methanol extracts 
      of the stems (EAkBs) and leaves (EAkBl) of AkB on human prostate cancer PC-3 
      cells. The antioxidant effects of EAkBs and EAkBl were measured using in vitro 
      total flavonoid and total phenolic assays and a free radical scavenging assay. 
      The effects of EAkBl on cell viability, apoptosis, autophagy, intracellular 
      reactive oxygen species (ROS) generation and protein expression levels were also 
      investigated. EAkBl was found to induce apoptosis, autophagy, and intracellular 
      ROS generation in PC-3 cells. In terms of protein levels, EAkBl reduced phospho 
      (p)-protein kinase B (AKT)/AKT, p-mammalian target of rapamycin (mTOR)/mTOR, 
      B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein (Bax) ratios, and the 
      activations of beclin 1/beta-actin and microtubule-associated protein 1A/1B-light 
      chain 3 (LC3) II/LC3 I ratios in PC-3 cells. The results of this study indicate 
      EAkBl has antioxidant and anticancer effects on prostate cancer cells, and that 
      these effects are associated with suppressions of p-AKT, p-mTOR, Bcl-2, and Bax, 
      and the activations of beclin 1 and LC3. Our results indicate EAkBl has potential 
      as a treatment for prostate cancer.
CI  - Copyright (c) 2021 China Pharmaceutical University. Published by Elsevier B.V. All 
      rights reserved.
FAU - Lee, Se-Eun
AU  - Lee SE
AD  - School of Korean Medicine, Pusan National University, Yangsan-si 50612, Republic 
      of Korea.
FAU - Sivtseva, Sardana
AU  - Sivtseva S
AD  - Department of Biology, North-Eastern Federal University, Yakutsk 677-027, Russia.
FAU - Lim, Chiyeon
AU  - Lim C
AD  - College of Medicine, Dongguk University, Gyeonggi-do 10326, Republic of Korea.
FAU - Okhlopkova, Zhanna
AU  - Okhlopkova Z
AD  - Department of Biology, North-Eastern Federal University, Yakutsk 677-027, Russia. 
      Electronic address: biotechnologyysu@rambler.ru.
FAU - Cho, Suin
AU  - Cho S
AD  - School of Korean Medicine, Pusan National University, Yangsan-si 50612, Republic 
      of Korea. Electronic address: sicho@pusan.ac.kr.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Nat Med
JT  - Chinese journal of natural medicines
JID - 101504416
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Apoptosis
MH  - *Artemisia/chemistry
MH  - *Autophagic Cell Death
MH  - Cell Line, Tumor
MH  - Humans
MH  - Male
MH  - *Plant Extracts/pharmacology
MH  - Plant Leaves/chemistry
MH  - *Prostatic Neoplasms/drug therapy/pathology
OTO - NOTNLM
OT  - Artemisia kruhsiana
OT  - Autophagy
OT  - Pharmacognosy
OT  - Prostate cancer
EDAT- 2021/03/02 06:00
MHDA- 2021/10/21 06:00
CRDT- 2021/03/01 05:28
PHST- 2020/04/10 00:00 [received]
PHST- 2021/03/01 05:28 [entrez]
PHST- 2021/03/02 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
AID - S1875-5364(21)60014-6 [pii]
AID - 10.1016/S1875-5364(21)60014-6 [doi]
PST - ppublish
SO  - Chin J Nat Med. 2021 Feb;19(2):134-142. doi: 10.1016/S1875-5364(21)60014-6.