PMID- 33643342
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210303
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Mitochondrial Small Heat Shock Proteins Are Essential for Normal Growth of 
      Arabidopsis thaliana.
PG  - 600426
LID - 10.3389/fpls.2021.600426 [doi]
LID - 600426
AB  - Mitochondria play important roles in the plant stress responses and the 
      detoxification of the reactive oxygen species generated in the electron transport 
      chain. Expression of genes encoding stress-related proteins such as the 
      mitochondrial small heat shock proteins (M-sHSP) is upregulated in response to 
      different abiotic stresses. In Arabidopsis thaliana, three M-sHSPs paralogous 
      genes were identified, although their function under physiological conditions 
      remains elusive. The aim of this work is to uncover the in vivo function of all 
      three M-sHSPs at the whole plant level. To accomplish this goal, we analyzed the 
      phenotype, proteomic, and metabolic profiles of Arabidopsis knock-down lines of 
      M-sHSPs (single, double, and triple knock-down lines) during normal plant growth. 
      The triple knock-down plants showed the most prominent altered phenotype at 
      vegetative and reproductive stages without any externally applied stress. They 
      displayed chlorotic leaves, growth arrest, and low seed production. 
      Concomitantly, they exhibited increased levels of sugars, proline, and citric, 
      malic, and ascorbic acid, among other metabolites. In contrast, single and double 
      knock-down plants displayed a few changes in their phenotype. A redundant 
      function among the three M-sHSPs is indicated by the impairment in vegetative and 
      reproductive growth associated with the simultaneous loss of all three M-sHSPs 
      genes. The triple knock-down lines showed alteration of proteins mainly involved 
      in photosynthesis and antioxidant defense compared to the control plants. On the 
      other hand, heat stress triggered a distinct cytosolic response pattern and the 
      upregulation of other sHSP members, in the knock-down plants. Overall, depletion 
      of all three M-sHSPs in Arabidopsis severely impacted fundamental metabolic 
      processes, leading to alterations in the correct plant growth and development. 
      These findings expand our knowledge about the contribution of organelle-specific 
      M-sHSPs to healthy plant growth under non-stress conditions.
CI  - Copyright (c) 2021 Escobar, Feussner and Valle.
FAU - Escobar, Mariela R
AU  - Escobar MR
AD  - Instituto de Biologia Molecular y Celular de Rosario (IBR-CONICET-UNR), Rosario, 
      Argentina.
FAU - Feussner, Ivo
AU  - Feussner I
AD  - Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant 
      Sciences, Gottingen Center for Molecular Biosciences (GZMB), University of 
      Gottingen, Gottingen, Germany.
FAU - Valle, Estela M
AU  - Valle EM
AD  - Instituto de Biologia Molecular y Celular de Rosario (IBR-CONICET-UNR), Rosario, 
      Argentina.
LA  - eng
PT  - Journal Article
DEP - 20210210
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7902927
OTO - NOTNLM
OT  - growth arrest
OT  - heat stress
OT  - metabolism
OT  - proteomics
OT  - sHSP
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/03/02 06:00
MHDA- 2021/03/02 06:01
PMCR- 2021/01/01
CRDT- 2021/03/01 05:36
PHST- 2020/08/30 00:00 [received]
PHST- 2021/01/04 00:00 [accepted]
PHST- 2021/03/01 05:36 [entrez]
PHST- 2021/03/02 06:00 [pubmed]
PHST- 2021/03/02 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.600426 [doi]
PST - epublish
SO  - Front Plant Sci. 2021 Feb 10;12:600426. doi: 10.3389/fpls.2021.600426. 
      eCollection 2021.