PMID- 33645033
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20211204
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 46
IP  - 3
DP  - 2021 Feb
TI  - [Effect of Gancao Fuzi Decoction on osteoarthritis and proteomics of articular 
      cartilage in rats].
PG  - 661-669
LID - 10.19540/j.cnki.cjcmm.20201210.401 [doi]
AB  - The rat osteoarthritis model was replicated by injection of sodium iodoacetate 
      into the knee joint cavity, and the effects of Gancao Fuzi Decoction on rat 
      osteoarthritis and the proteome of articular cartilage were investigated. Sixty 
      SD rats weighing 230-250 g were randomly divided into normal group, model group, 
      glucosamine sulfate group, and Gancao Fuzi Decoction high, medium and low dose 
      groups. Osteoarthritis model was induced by intra-articular injection of sodium 
      iodoacetate(3 mg on each leg) in all groups except the normal group. After 
      modeling, each administration group was given intragastric administration for 1 
      month. During the administration period, joint pain test and joint width 
      measurement were performed every week to observe the autonomous behavior of rats. 
      Enzyme linked immunosorbent assay(ELISA) method was used to detect the contents 
      of tumor necrosis factor-alpha(TNF-alpha), interleukin-1beta(IL-1beta), matrix 
      metalloproteinase-3(MMP-3), and matrix metalloproteinase tissue inhibitor(TIMP-1) 
      in rat joint lavage fluid. Hematoxylin-eosin(HE) staining was used to observe 
      bone and joint morphology. Nano-LC-LTQ-Orbitrap system was used to detect 
      arti-cular cartilage proteins. The results showed that, compared with the model 
      group, Gancao Fuzi Decoction could significantly improve joint pain and joint 
      swelling in osteoarthritis rats, significantly reduce the contents of TNF-alpha, 
      IL-1beta and MMP-3 in the joint cavity la-vage fluid, increase the content of 
      TIMP-1, and relieve inflammatory diseases such as enlarged joint space, rough 
      cartilage edge, different thickness of cartilage layer, and disordered 
      arrangement of chondrocytes. After comparing the proteins between the groups, 273 
      differential proteins were screened out. KEGG analysis found that the above 
      differential proteins involved 43 signaling pathways such as systemic lupus 
      erythematosus, among which 11 signaling pathways were related to osteoarthritis. 
      The above results indicated that Gancao Fuzi Decoction had a preventive effect on 
      osteoarthritis, and its mechanism of action may be accomplished by regulating the 
      protein expression of osteoarthritis-related signal pathways.
FAU - Huang, Si-Min
AU  - Huang SM
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
FAU - Wu, Yu-Meng
AU  - Wu YM
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
FAU - Xie, Hui-Hui
AU  - Xie HH
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
FAU - Yang, Shuo
AU  - Yang S
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
FAU - Xu, Peng
AU  - Xu P
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
FAU - Liu, Bo
AU  - Liu B
AD  - School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 
      330004, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Diterpenes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Extracts)
RN  - 0 (fuzi drug herbal)
RN  - 2788Z9758H (glycyrrhizae radix et rhizoma)
SB  - IM
MH  - Animals
MH  - *Cartilage, Articular
MH  - Diterpenes
MH  - Drugs, Chinese Herbal
MH  - Glycyrrhiza
MH  - *Osteoarthritis/drug therapy
MH  - Plant Extracts
MH  - Proteomics
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Gancao Fuzi Decoction
OT  - osteoarthritis
OT  - proteomics
OT  - sodium iodoacetate
EDAT- 2021/03/02 06:00
MHDA- 2021/03/03 06:00
CRDT- 2021/03/01 05:46
PHST- 2021/03/01 05:46 [entrez]
PHST- 2021/03/02 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20201210.401 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2021 Feb;46(3):661-669. doi: 
      10.19540/j.cnki.cjcmm.20201210.401.