PMID- 33645563
OWN - NLM
STAT- MEDLINE
DCOM- 20210318
LR  - 20210416
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 168
DP  - 2021 Feb 11
TI  - In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste 
      Stimuli.
LID - 10.3791/62172 [doi]
AB  - Within the last ten years, advances in genetically encoded calcium indicators 
      (GECIs) have promoted a revolution in in vivo functional imaging. Using calcium 
      as a proxy for neuronal activity, these techniques provide a way to monitor the 
      responses of individual cells within large neuronal ensembles to a variety of 
      stimuli in real time. We, and others, have applied these techniques to image the 
      responses of individual geniculate ganglion neurons to taste stimuli applied to 
      the tongues of live anesthetized mice. The geniculate ganglion is comprised of 
      the cell bodies of gustatory neurons innervating the anterior tongue and palate 
      as well as some somatosensory neurons innervating the pinna of the ear. Imaging 
      the taste-evoked responses of individual geniculate ganglion neurons with GCaMP 
      has provided important information about the tuning profiles of these neurons in 
      wild-type mice as well as a way to detect peripheral taste miswiring phenotypes 
      in genetically manipulated mice. Here we demonstrate the surgical procedure to 
      expose the geniculate ganglion, GCaMP fluorescence image acquisition, initial 
      steps for data analysis, and troubleshooting. This technique can be used with 
      transgenically encoded GCaMP, or with AAV-mediated GCaMP expression, and can be 
      modified to image particular genetic subsets of interest (i.e., Cre-mediated 
      GCaMP expression). Overall, in vivo calcium imaging of geniculate ganglion 
      neurons is a powerful technique for monitoring the activity of peripheral 
      gustatory neurons and provides complementary information to more traditional 
      whole-nerve chorda tympani recordings or taste behavior assays.
FAU - Fowler, Bryan E
AU  - Fowler BE
AD  - Department of Biology, The University of Texas at San Antonio.
FAU - Macpherson, Lindsey J
AU  - Macpherson LJ
AD  - Department of Biology, The University of Texas at San Antonio; 
      lindsey.macpherson@utsa.edu.
LA  - eng
GR  - SC2 GM130411/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20210211
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Anesthesia
MH  - Animals
MH  - Calcium/*metabolism
MH  - Geniculate Ganglion/*physiology/surgery
MH  - Immobilization
MH  - Mice
MH  - Neurons/*physiology
MH  - Physical Stimulation
MH  - Taste/*physiology
MH  - Taste Buds/physiology
MH  - Tracheotomy
PMC - PMC8048314
MID - NIHMS1684544
COIS- The authors have no conflict of interest to report.
EDAT- 2021/03/02 06:00
MHDA- 2021/03/19 06:00
PMCR- 2021/04/15
CRDT- 2021/03/01 09:22
PHST- 2021/03/01 09:22 [entrez]
PHST- 2021/03/02 06:00 [pubmed]
PHST- 2021/03/19 06:00 [medline]
PHST- 2021/04/15 00:00 [pmc-release]
AID - 10.3791/62172 [doi]
PST - epublish
SO  - J Vis Exp. 2021 Feb 11;(168):10.3791/62172. doi: 10.3791/62172.