PMID- 33647765
OWN - NLM
STAT- MEDLINE
DCOM- 20211013
LR  - 20211013
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 44
IP  - 2
DP  - 2021 Apr
TI  - Flavobacterium turcicum sp. nov. and Flavobacterium kayseriense sp. nov. isolated 
      from farmed rainbow trout in Turkey.
PG  - 126186
LID - S0723-2020(21)00009-6 [pii]
LID - 10.1016/j.syapm.2021.126186 [doi]
AB  - During a study on culturable microorganisms from fish farms, four 
      yellow-pigmented gram negative, rod shaped isolates, F-47(T), F-339(T), F-380 and 
      F-400, were recovered from rainbow trout samples exhibiting clinical signs. Based 
      on 16S rRNA gene sequence analysis, the strains were identified as members of the 
      genus Flavobacterium. Strains F-47(T) and F-380 shared the highest 16S rRNA gene 
      sequence identity level of 97.6% with the type strain of Flavobacterium frigoris 
      DSM 15719(T) while strains F-339(T) and F-400 shared the highest identity level 
      of 97.6% with the type strain of F. caseinilyticum AT-3-2(T). A polyphasic 
      taxonomic approach including phenotypic and genomic characterization as well as 
      whole-cell MALDI-TOF mass spectrometry analyses was employed to ascertain the 
      taxonomic position of the strains within the genus Flavobacterium. Digital 
      DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses 
      between strains F-47(T), F-339(T) and their close neighbours F. frigoris DSM 
      15719(T) and F. caseinilyticum AT-3-2(T), respectively, confirmed that both 
      strains represent novel species in the genus Flavobacterium. The DNA G+C contents 
      of the strains F-47(T) and F-339(T) are 34.3% and 35.3%, respectively. It can be 
      concluded on the basis of polyphasic characterization as well as pairwise genome 
      comparisons that the strains F-47(T) and F-339(T) represent two novel species 
      within the genus Flavobacterium, for which Flavobacterium kayseriense sp. nov. 
      F-47(T) (=JCM 34195(T)=KCTC 82255(T)) and Flavobacterium turcicum sp. nov. 
      F-339(T) (=JCM 34202(T)=KCTC 82262(T)) are proposed, respectively.
CI  - Copyright (c) 2021 Elsevier GmbH. All rights reserved.
FAU - Saticioglu, Izzet Burcin
AU  - Saticioglu IB
AD  - Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Erciyes 
      University, 38280, Kayseri, Turkey. Electronic address: iburcinsat@gmail.com.
FAU - Ay, Hilal
AU  - Ay H
AD  - Department of Molecular Biology and Genetics, Faculty of Science and Arts, 
      Ondokuz Mayis University, 55139 Samsun, Turkey.
FAU - Altun, Soner
AU  - Altun S
AD  - Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa 
      Uludag University, Bursa 16059, Turkey.
FAU - Duman, Muhammed
AU  - Duman M
AD  - Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa 
      Uludag University, Bursa 16059, Turkey.
FAU - Sahin, Nevzat
AU  - Sahin N
AD  - Department of Molecular Biology and Genetics, Faculty of Science and Arts, 
      Ondokuz Mayis University, 55139 Samsun, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20210220
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacterial Typing Techniques
MH  - Base Composition
MH  - DNA, Bacterial/genetics
MH  - Flavobacterium/*classification/isolation & purification
MH  - Nucleic Acid Hybridization
MH  - *Oncorhynchus mykiss/microbiology
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Turkey
OTO - NOTNLM
OT  - Aquaculture
OT  - Flavobacterium
OT  - Oncorhynchus mykiss
OT  - Rainbow trout
EDAT- 2021/03/02 06:00
MHDA- 2021/10/14 06:00
CRDT- 2021/03/01 20:18
PHST- 2021/01/09 00:00 [received]
PHST- 2021/02/04 00:00 [revised]
PHST- 2021/02/08 00:00 [accepted]
PHST- 2021/03/02 06:00 [pubmed]
PHST- 2021/10/14 06:00 [medline]
PHST- 2021/03/01 20:18 [entrez]
AID - S0723-2020(21)00009-6 [pii]
AID - 10.1016/j.syapm.2021.126186 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2021 Apr;44(2):126186. doi: 10.1016/j.syapm.2021.126186. 
      Epub 2021 Feb 20.