PMID- 33649112
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Print)
IS  - 0066-4804 (Linking)
VI  - 65
IP  - 5
DP  - 2021 May 1
TI  - in vitro Activity of Hydrogen Peroxide and Hypochlorous Acid Generated by 
      Electrochemical Scaffolds Against Planktonic and Biofilm Bacteria.
LID - AAC.01966-20 [pii]
LID - 10.1128/AAC.01966-20 [doi]
LID - e01966-20
AB  - Hydrogen peroxide (H(2)O(2)) and hypochlorous acid (HOCl) are biocides used for 
      cleaning and debriding chronic wound infections, which often harbor drug 
      resistant bacteria. Here, we evaluated the in vitro activity of H(2)O(2) and HOCl 
      against 27 isolates of eight bacterial species involved in wound infections. 
      Minimum inhibitory concentrations (MICs) and minimum biofilm bactericidal 
      concentrations (MBBCs) were measured. When compared to their respective MICs, 
      MBBCs of isolates exposed to H(2)O(2) were 16- to 1,024-fold higher and those 
      exposed to HOCl were 2- to 4-fold higher. We evaluated selection of resistance 
      after exposure of Staphylococcus aureus and Pseudomonas aeruginosa biofilms to 10 
      iterations of electrochemically generated HOCl or H(2)O(2) delivered using 
      electrochemical scaffolds (e-scaffolds), observing no decrease in anti-biofilm 
      effects with serial exposure to e-scaffold-generated H(2)O(2) or HOCl. 24-hour 
      exposure to H(2)O(2)-generating e-scaffolds consistently decreased colony forming 
      units (CFUs) of S. aureus and P. aeruginosa biofilms by  approximately 5.0-log(10) and 
       approximately 4.78-log(10) through 10 iterations of exposure, respectively. 4-hour exposure to 
      HOCl-generating e-scaffolds consistently decreased CFUs of S. aureus biofilms by 
       approximately 4.9-log(10), and 1-hour exposure to HOCl-generating e-scaffolds consistently 
      decreased CFUs of P. aeruginosa biofilms by  approximately 1.57-log(10) These results suggest 
      that HOCl has similar activity against planktonic and biofilm bacteria, whereas 
      the activity of H(2)O(2) is less against biofilm than planktonic bacteria, and 
      that repeat exposure to either biocide, generated electrochemically under the 
      experimental conditions studied, does not lessen antibiofilm effects.
CI  - Copyright (c) 2021 American Society for Microbiology.
FAU - Raval, Yash S
AU  - Raval YS
AUID- ORCID: 0000-0003-1161-4000
AD  - Division of Clinical Microbiology, Mayo Clinic, Rochester, MN.
FAU - Flurin, Laure
AU  - Flurin L
AUID- ORCID: 0000-0001-5687-1081
AD  - Division of Clinical Microbiology, Mayo Clinic, Rochester, MN.
FAU - Mohamed, Abdelrhman
AU  - Mohamed A
AUID- ORCID: 0000-0003-2132-0487
AD  - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, 
      Washington State University, Pullman, WA.
FAU - Greenwood-Quaintance, Kerryl E
AU  - Greenwood-Quaintance KE
AD  - Division of Clinical Microbiology, Mayo Clinic, Rochester, MN.
FAU - Beyenal, Haluk
AU  - Beyenal H
AD  - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, 
      Washington State University, Pullman, WA.
FAU - Patel, Robin
AU  - Patel R
AUID- ORCID: 0000-0001-6344-4141
AD  - Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 
      patel.robin@mayo.edu.
AD  - Division of Infectious Diseases, Mayo Clinic, Rochester, MN.
LA  - eng
GR  - R01 AI091594/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20210301
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
SB  - IM
PMC - PMC8092879
EDAT- 2021/03/03 06:00
MHDA- 2021/03/03 06:01
PMCR- 2021/10/19
CRDT- 2021/03/02 06:09
PHST- 2021/03/02 06:09 [entrez]
PHST- 2021/03/03 06:00 [pubmed]
PHST- 2021/03/03 06:01 [medline]
PHST- 2021/10/19 00:00 [pmc-release]
AID - AAC.01966-20 [pii]
AID - 01966-20 [pii]
AID - 10.1128/AAC.01966-20 [doi]
PST - ppublish
SO  - Antimicrob Agents Chemother. 2021 May 1;65(5):e01966-20. doi: 
      10.1128/AAC.01966-20. Epub 2021 Mar 1.