PMID- 33650104
OWN - NLM
STAT- MEDLINE
DCOM- 20210909
LR  - 20210909
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 101
IP  - 13
DP  - 2021 Oct
TI  - Effect of roasted peanut allergen Ara h 3 protein on the sensitization of Caco-2 
      cells.
PG  - 5325-5336
LID - 10.1002/jsfa.11180 [doi]
AB  - BACKGROUND: Roasted peanut is widely loved as a kind of food with rich taste. 
      However, peanut allergy is one of the major threats to human health, which 
      affects about 5% of children and 1.4-2% of adults in the world. RESULTS: To 
      evaluate the sensitization mechanism of peanut allergen Ara h 3, Caco-2 cells as 
      the model, which has the similar structure and function to differentiated small 
      intestinal epithelial cells. Compared with Ara h 3-raw (purified from raw peanut) 
      group, more significant results such as the inhibited Caco-2 cell viability and 
      proliferation, the increased secretion of reactive oxygen species (ROS) and the 
      decreased transepithelial electrical resistance were obtained in Ara h 3-roasted 
      (purified from roasted peanut) group. Accordingly, oxidative stress and NF-kappaB 
      signaling pathway were more imbalanced, which lead to the increased of thymic 
      stromal lymphopoietin (TSLP), interleukin 6 (IL-6), IL-8 and monocyte chemotactic 
      protein 1 (MCP-1). Then, the gene expression of tight junction proteins ZO-1, 
      occludin and JAM-1 were reduced, which proved that the integrity of the Caco-2 
      monolayer barrier is severely damaged. CONCLUSION: These finding identify the 
      mechanisms of the allergenicity of roasted peanut allergy proteins are probably 
      associated with intestinal uptake and cytokine dependent allergies. The 
      aggravated allergic reaction might be caused by the increment of TSLP, IL-6, IL-8 
      and MCP-1 due to the activated NF-kappaB signaling pathway, and the enhanced 
      transport of Ara h 3-roasted protein by Caco-2 monolayer. (c) 2021 Society of 
      Chemical Industry.
CI  - (c) 2021 Society of Chemical Industry.
FAU - Wang, Shuo
AU  - Wang S
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
FAU - Sun, Xiaodong
AU  - Sun X
AD  - School of Medicine, Shanghai University, Shanghai, China.
FAU - Wang, Minjia
AU  - Wang M
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
FAU - Deng, Zhirui
AU  - Deng Z
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
FAU - Niu, Bing
AU  - Niu B
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
FAU - Chen, Qin
AU  - Chen Q
AUID- ORCID: 0000-0003-3089-9195
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
LA  - eng
GR  - 19395810100/Science and Technology Joint Project of the Yangtze River Delta/
GR  - 19395810100/Central Guidance for Local Science and Technology Development/
PT  - Journal Article
DEP - 20210326
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h 3 allergen, Arachis hypogea)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (F11R protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Zonula Occludens-1 Protein)
SB  - IM
MH  - Allergens/chemistry/immunology
MH  - Antigens, Plant/chemistry/*immunology
MH  - Arachis/chemistry/*immunology
MH  - Caco-2 Cells
MH  - Cell Adhesion Molecules/immunology
MH  - Chemokine CCL2/immunology
MH  - Epithelial Cells/*immunology
MH  - Humans
MH  - Interleukin-6/immunology
MH  - Interleukin-8/immunology
MH  - Intestine, Small/immunology
MH  - NF-kappa B/immunology
MH  - Peanut Hypersensitivity/*immunology
MH  - Plant Proteins/chemistry/*immunology
MH  - Receptors, Cell Surface/immunology
MH  - Seeds/chemistry/immunology
MH  - Zonula Occludens-1 Protein/immunology
OTO - NOTNLM
OT  - Caco-2 cells
OT  - NF-kappaB signaling pathway
OT  - inflammatory factor
OT  - oxidative stress
OT  - roasted peanut
EDAT- 2021/03/03 06:00
MHDA- 2021/09/10 06:00
CRDT- 2021/03/02 06:24
PHST- 2021/02/23 00:00 [revised]
PHST- 2020/08/18 00:00 [received]
PHST- 2021/03/01 00:00 [accepted]
PHST- 2021/03/03 06:00 [pubmed]
PHST- 2021/09/10 06:00 [medline]
PHST- 2021/03/02 06:24 [entrez]
AID - 10.1002/jsfa.11180 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2021 Oct;101(13):5325-5336. doi: 10.1002/jsfa.11180. Epub 2021 
      Mar 26.