PMID- 33651350
OWN - NLM
STAT- MEDLINE
DCOM- 20210405
LR  - 20210405
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2292
DP  - 2021
TI  - Fluorescence In Situ Hybridization in Urine Samples (UroVysion Kit).
PG  - 35-48
LID - 10.1007/978-1-0716-1354-2_4 [doi]
AB  - Cystoscopy is considered the standard approach to the diagnostic workup of 
      urinary symptoms. It has high sensitivity and specificity for papillary tumors of 
      the bladder but low sensitivity and specificity for flat lesions. It is also 
      expensive and may cause discomfort and complications. Urine cytology, in 
      contrast, has the advantage of being a noninvasive test with high specificity but 
      suffers from low sensitivity in low-grade and early-stage tumors, possibly due to 
      the low number of exfoliated cells in urine. Numerous new noninvasive tests have 
      been proposed. Among these, fluorescence in situ hybridization (FISH) has been 
      studied for long time and in 2005 UroVysion Bladder Cancer Kit (UroVysion Kit) 
      (Abbott/Vysis) received FDA approval for initial diagnosis of bladder carcinoma 
      in patients with hematuria and subsequent monitoring for tumor recurrence in 
      patients previously diagnosed with bladder cancer. The UroVysion Kit is designed 
      to detect aneuploidy for chromosomes 3, 7, 17, and loss of the 9p21 locus by FISH 
      in urine specimens from symptomatic patients, those with hematuria suspected of 
      having bladder cancer. Here, the approach for FISH assay by using UroVysion 
      Bladder Cancer kit according to manufacturer's instructions is described.
FAU - Bravaccini, Sara
AU  - Bravaccini S
AD  - Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura 
      dei Tumori (IRST) IRCCS, Meldola, Italy. sara.bravaccini@irst.emr.it.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Aneuploidy
MH  - Chromosomes, Human/genetics
MH  - Hematuria/genetics/*urine
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Urinary Bladder Neoplasms/genetics/*urine
OTO - NOTNLM
OT  - Bladder cancer
OT  - Chromosomes 3, 7, 9, and 17
OT  - Fluorescence in situ hybridization
OT  - UroVysion
OT  - Voided urine
EDAT- 2021/03/03 06:00
MHDA- 2021/04/07 06:00
CRDT- 2021/03/02 12:15
PHST- 2021/03/02 12:15 [entrez]
PHST- 2021/03/03 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
AID - 10.1007/978-1-0716-1354-2_4 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2292:35-48. doi: 10.1007/978-1-0716-1354-2_4.